| 研究課題/領域番号 |
20K16271
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分49070:免疫学関連
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| 研究機関 | 北海道大学 |
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研究代表者 |
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| 研究期間 (年度) |
2020-04-01 – 2021-03-31
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| 研究課題ステータス |
中途終了 (2020年度)
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| 配分額 *注記 |
4,160千円 (直接経費: 3,200千円、間接経費: 960千円)
2021年度: 2,080千円 (直接経費: 1,600千円、間接経費: 480千円)
2020年度: 2,080千円 (直接経費: 1,600千円、間接経費: 480千円)
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| キーワード | Crohn's Disease / NOD2 / HD-5 / RNASeq / Microbiome |
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| 研究開始時の研究の概要 |
(1) Characterize the host tissue and microbial dysbiotic changes that precipitate CD under Nod2-deficiency and (2) validate the preclinical use of an anti-microbial peptide, human defensin-5, to supplement Nod2-deficiency dependent impairment of Paneth cell function to prevent and/or rescue CD.
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| 研究実績の概要 |
In oder to elucidate the function of Nod2 and dysbiosis in the ileum we set out the followings during the first year:
1) establishing in vitro analysis of Paneth cell function for bactericidal activity. We established the in vitro system to isolate Paneth cells, which is ready to secrete alpha defensins upon ligand stimulation. The molecular mechanisms by which Nod2 regulate the section will be sought out at the second year.
2) establishing real time secretion assay of Paneth cells. With Dr. Ayabe's group collaboration, we set up real-time imaging system which provides robust information as to alpha defensin secretion through real-time visualization. The system will be applied to the Paneth cells derived from wild-type, Nod2- or Rip2-deficient mice at the second year.
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