| 研究課題/領域番号 |
20K23057
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| 研究種目 |
研究活動スタート支援
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| 配分区分 | 基金 |
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| 審査区分 |
0907:口腔科学およびその関連分野
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| 研究機関 | 国立研究開発法人国立長寿医療研究センター (2021)
徳島大学 (2020) |
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研究代表者 |
ラジュ レスミ 国立研究開発法人国立長寿医療研究センター, ジェロサイエンス研究センター, 研究員 (40876702)
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| 研究期間 (年度) |
2020-09-11 – 2022-03-31
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| 研究課題ステータス |
完了 (2021年度)
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| 配分額 *注記 |
2,730千円 (直接経費: 2,100千円、間接経費: 630千円)
2021年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
2020年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
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| キーワード | regeneration / tissue engineering / periodontal tissue / tooth regeneration / Periodontal tissue / Regenerative medicine / Tissue engineering |
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| 研究開始時の研究の概要 |
Periodontal tissue is composed of cementum, periodontal ligament (PDL) and alveolar bone, given the complexity of this redundant structure, a single type of cell is not enough to regenerate such combined tissue. Here we are developing a complex cell sheet composed of PDL and osteogenic differentiated PDL cells. Furthermore, we will be analyzing the regenerative capacity of this cell sheet by ectopic and orthotopic transplantation in mice.
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| 研究実績の概要 |
Complex cell sheet, CCS was made using rat PDL cells and osteogenic differentiated PDL cells. Transplants from ectopic transplantation analysis using histology and micro-CT showed the formation of periodontal ligament like bone like tissue. Furthermore, we have successfully created an orthotropic injury mice model for the transplantation of cell sheets. In a continuation study, we have evaluated the regenerative capacity of tooth dentin-pulp tissue by a CCS. Dental pulp stem cells, DPSCs and odontogenic differentiated DPSCs were utilized to fabricate a CCS. Ectopic transplants after 8 weeks of transplantation revealed the formation of dentin-pulp like combined hard and soft tissue formation.
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