分裂酵母における減数分裂の制御機構

• 研究課題/領域番号: 21227007
• 研究種目: 基盤研究(S)
• 配分区分: 補助金
• 研究分野: 細胞生物学
• 研究機関: 公益財団法人かずさＤＮＡ研究所 (2012-2013); 東京大学 (2009-2011)
• 研究代表者: 山本 正幸 公益財団法人かずさＤＮＡ研究所, その他部局等, 特別客員研究員 (40114706)
• 連携研究者: 山下 朗 かずさDNA研究所, ヒトゲノム研究部, 室長 (30312276) ; 佐藤 政充 早稲田大学, 先進理工学部, 准教授 (50447356)
• 研究期間 (年度): 2009-05-11 – 2014-03-31
• 研究課題ステータス: 完了 (2013年度)
• 配分額: 207,740千円 (直接経費: 159,800千円、間接経費: 47,940千円); 2013年度: 27,820千円 (直接経費: 21,400千円、間接経費: 6,420千円); 2012年度: 27,820千円 (直接経費: 21,400千円、間接経費: 6,420千円); 2011年度: 46,800千円 (直接経費: 36,000千円、間接経費: 10,800千円); 2010年度: 46,800千円 (直接経費: 36,000千円、間接経費: 10,800千円); 2009年度: 58,500千円 (直接経費: 45,000千円、間接経費: 13,500千円)
• キーワード: 遺伝学 / 発生・分化 / 減数分裂 / 情報伝達 / RNA / 微小管

研究概要

分裂酵母を用いて減数分裂を制御する分子機構を調べた。減数分裂に必要な一群のmRNAは栄養増殖時の細胞では「選択的除去」とよぶRNA分解を受ける。この機構を解析し、関係する諸分子を明らかにした。中心因子であるMmi1タンパク質は、標的mRNA上の特定の６塩基配列を認識し結合した。減数分裂時にMmi1を不活化させるmeiRNAにはこの６塩基配列が多数存在し、Mmi1をおびき寄せていた。また、RNA polymerase II のC末端領域のSer-2のリン酸化が減数分裂開始に必要な特定遺伝子の転写に不可欠なこと、ストレスに応答するMAPキナーゼ経路がそこ介在することを解明した。

評価記号

検証結果 (区分)

A+

研究成果

• [雑誌論文] S. pombe TOR complex 1 activates the ubiquitin-proteasomal degradation of the meiotic regulator Mei2 in cooperation with Pat1 kinase (2014)
  • 著者名/発表者名: Y. Otsubo, A. Yamashita, H. Ohno and M. Yamamoto
  • 雑誌名: J. Cell Sci 巻: (in press)
  • 査読あり
• [雑誌論文] CDK-dependent phosphorylation of Alp7-Alp14 (TACC-TOG) promotes its nuclear accumulation and spindle microtubule assembly (2014)
  • 著者名/発表者名: N. Okada, T. Toda, M. Yamamoto and M. Sato
  • 雑誌名: Mol. Biol. Cell 巻: (in press)
  • 査読あり
• [雑誌論文] Meiotic long non-coding meiRNA accumulates as a dot at its genetic locus facilitated by Mmi1 and plays as a decoy to lure Mmi1 (2014)
  • 著者名/発表者名: Y. Shichino, A. Yamashita and M. Yamamoto
  • 雑誌名: Open Biol 巻: 4 号: 6 ページ: 140022-140022
  • DOI: 10.1098/rsob.140022
  • 査読あり
• [雑誌論文] The RNA-binding protein Spo5 promotes meiosis II by regulating cyclin Cdc13 in fission yeast (2014)
  • 著者名/発表者名: M. Arata, M. Sato, A. Yamashita and M. Yamamoto
  • 雑誌名: Genes Cells 巻: 19 ページ: 225-238
  • 査読あり
• [雑誌論文] The RNA-binding protein Spo5 promotes meiosis II by regulating cyclin Cdc13 in fission yeast. (2014)
  • 著者名/発表者名: Mayumi Arata
  • 雑誌名: Genes to Cells 巻: 19 号: 3 ページ: 225-238
  • DOI: 10.1111/gtc.12133
  • 査読あり
• [雑誌論文] S. pombe TOR complex 1 activates the ubiquitin-proteasomal degradation of the meiotic regulator Mei2 in cooperation with Pat1 kinase. (2014)
  • 著者名/発表者名: Yoko Otsubo
  • 雑誌名: Journal of Cell Science 巻: 127
  • 査読あり
• [雑誌論文] CDK-dependent phosphorylation of Alp7-Alp14 (TACC-TOG) promotes its nuclear accumulation and spindle microtubule assembly. (2014)
  • 著者名/発表者名: Naoyuki Okada
  • 雑誌名: Molecular Biology of the Cell 巻: 25
  • 査読あり
• [雑誌論文] A novel factor Iss10 regulates Mmi1-mediated selective elimination of meiotic transcripts (2013)
  • 著者名/発表者名: A. Yamashita, T. Takayama, R. Iwata and M. Yamamoto
  • 雑誌名: Nucleic Acids Res 巻: 41 ページ: 9680-9687
  • 査読あり
• [雑誌論文] Microtubules and Alp7-Alp14 (TACC-TOG) reposition chromosomes before meiotic segregation (2013)
  • 著者名/発表者名: Y. Kakui, M. Sato, N. Okada, T. Toda and M. Yamamoto
  • 雑誌名: Nat. Cell Biol 巻: 15 ページ: 786-796
  • 査読あり
• [雑誌論文] The fission yeast ・-arrestin-like protein Any1 is involved in TSC-Rheb signaling and the regulation of amino acid transporters (2013)
  • 著者名/発表者名: Y. Nakase, M. Nakase, J. Kashiwazaki, T. Murai, Y. Otsubo, I. Mabuchi, M. Yamamoto, K. Takegawa and T. Matsumoto
  • 雑誌名: J. Cell Sci 巻: 126 ページ: 3972-3981
  • 査読あり
• [雑誌論文] Cuf2 boosts the transcription of APC/C activator Fzr1 to terminate the meiotic division cycle (2013)
  • 著者名/発表者名: Y. Aoi, K. Arai, M. Miyamoto, Y. Katsuta, A. Yamashita, M. Sato and M. Yamamoto
  • 雑誌名: EMBO Rep 巻: 14 ページ: 553-60
  • 査読あり
• [雑誌論文] Proper microtubule structure is vital for timely progression through meiosis in fission yeast (2013)
  • 著者名/発表者名: A. Yamashita, Y. Fujita and M. Yamamoto
  • 雑誌名: PLoS One 巻: 8 号: 6 ページ: e65082-e65082
  • DOI: 10.1371/journal.pone.0065082
  • 査読あり
• [雑誌論文] Cuf2 boosts the transcription of APC/C actlvator Fzri to terminate the meiotic division cycle. (2013)
  • 著者名/発表者名: Yuki Aoi, Kunio Arai, Masaya Miyamoto, Yuji Katsuta, Akira Yamashita, Masamitsu Sato, Masayuki Yamamoto.
  • 雑誌名: EMBO Reports 巻: 14 号: 6 ページ: 553-560
  • DOI: 10.1038/embor.2013.52
  • 査読あり
• [雑誌論文] Microtubules and Alp7-Alp14 (TACC-TOG) reposition chromosomes before meiotic segregation (2013)
  • 著者名/発表者名: Yasutaka Kakui, Masamitsu Sato, Naoyuki Okada, Takashi Toda, and Masayuki Yamamoto
  • 雑誌名: Nature Cell Biology 巻: 15 号: 7 ページ: 786-796
  • DOI: 10.1038/ncb2782
  • 査読あり
• [雑誌論文] Fission yeast Anyl, B-arrestin-like protein, is involved in TSC-Rheb signaling and the regulation or amino acid transporters. (2013)
  • 著者名/発表者名: Nakase Y, Nakase M, Kashiwazaki J, Murai T, Otsubo Y, Mabuchi I, Yamamoto M, Takegawa K and Matsumoto T.
  • 雑誌名: Journal of Cell Science 巻: 1;126 ページ: 3972-3981
  • DOI: 10.1242/jcs.128355
  • 査読あり / オープンアクセス
• [雑誌論文] A novel factor Iss10 regulates Mmi1-mediated selective elimination of meiotic transcripts. (2013)
  • 著者名/発表者名: Akira Yamashita
  • 雑誌名: Nucleic Acids Research 巻: 41 号: 21 ページ: 9680-9687
  • DOI: 10.1093/nar/gkt763
  • 査読あり
• [雑誌論文] Signaling pathways for fission yeast sexual differentiation at a glance (2012)
  • 著者名/発表者名: Y. Otsubo and M. Yamamoto
  • 雑誌名: J. Cell Sci 巻: 125 ページ: 2789-2793
  • 査読あり
• [雑誌論文] SPB components are reorganized during fission yeast meiosis (2012)
  • 著者名/発表者名: M. Ohta, M. Sato and M. Yamamoto
  • 雑誌名: Mol. Biol. Cell 巻: 23 ページ: 1799-1811
  • 査読あり
• [雑誌論文] Meiosis-specific non-coding RNA mediates robust pairing of homologous chromosomes in meiosis (2012)
  • 著者名/発表者名: D.-Q. Ding, K. Okamasa, M. Yamane, C. Tsutsumi, T. Haraguchi, M. Yamamoto and Y. Hiraoka
  • 雑誌名: Science 巻: 336 ページ: 732-736
  • 査読あり
• [雑誌論文] Mmi1 RNA surveillance machinery directs RNAi complex RITS to selective meiotic genes in fission yeast (2012)
  • 著者名/発表者名: E. Hiriart, A. Vavasseur, L. Touat- Todeschini, A. Yamashita, B. Gilquin, E. Lambert, J. Perot, Y. Shichino, N. Nazaret, C. Boyault, J. Lachuer, D. Perazza, M. Yamamoto and A. Verdel
  • 雑誌名: EMBO J 巻: 31 ページ: 2296-2308
  • 査読あり
• [雑誌論文] Psk1, an AGC kinase family member in fission yeast, is directly phosphorylated and controlled by TORC1 and functions as S6 kinase (2012)
  • 著者名/発表者名: A. Nakashima, Y. Otsubo, A. Yamashita, T. Sato, M. Yamamoto and F. Tamanoi
  • 雑誌名: J. Cell Sci 巻: 125 ページ: 5840-5849
  • 査読あり
• [雑誌論文] Transient structure associated with the spindle pole body directs meiotic microtubule reorganization in S. pombe (2012)
  • 著者名/発表者名: C. Funaya, S. Samarasinghe, S. Pruggnaller, M. Ohta, Y. Connolly, J. Muller, H. Murakami, A. Grallert, M. Yamamoto, D. Smith, C. Antony and K. Tanaka
  • 雑誌名: Curr. Biol 巻: 22 ページ: 562-574
  • 査読あり
• [雑誌論文] Interpolar microtubules are dispensable in fission yeast meiosis II (2012)
  • 著者名/発表者名: T. Akera, M. Sato and M. Yamamoto
  • 雑誌名: Nat. Commun 巻: 3 号: 1 ページ: 695-695
  • DOI: 10.1038/ncomms1725
  • 査読あり
• [雑誌論文] Hexanucleotide motifs mediate recruitment of the RNA elimination machinery to silent meiotic genes (2012)
  • 著者名/発表者名: Akira Yamashita, Yuichi Shichino, Hirotsugu Tanaka, Edwige Hiriart, Leila Touat-Todeschini, Aurelia Vavasseur, Da-Qiao Ding, Yasushi Hiraoka, Andre Verdel, Masayuki Yamamoto
  • 雑誌名: Open Biology 巻: 2 号: 3 ページ: 120014-120014
  • DOI: 10.1098/rsob.120014
  • 査読あり
• [雑誌論文] SCF ensures meiotic chromosome segregation through a resolution of meiotic recombination intermediates (2012)
  • 著者名/発表者名: S. Okamoto, M. Sato, T. Toda and M. Yamamoto
  • 雑誌名: PLoS One 巻: 7 号: 1 ページ: 30622-30622
  • DOI: 10.1371/journal.pone.0030622
  • 査読あり
• [雑誌論文] Transient structure associated with the spindle pole body directs meiotic microtubule reorganization in S. pombe. (2012)
  • 著者名/発表者名: Charlotta Funaya
  • 雑誌名: Current Biology 巻: 22 号: 7 ページ: 562-574
  • DOI: 10.1016/j.cub.2012.02.042
  • 査読あり
• [雑誌論文] SPB components are reorganized during fission yeast meiosis. (2012)
  • 著者名/発表者名: Midori Ohta
  • 雑誌名: Molecular Biology of the Cell 巻: 23 号: 10 ページ: 1799-1811
  • DOI: 10.1091/mbc.e11-11-0951
  • 査読あり
• [雑誌論文] Mmil RNA surveillance machinery directs RNAi complex RITS to specific meiotic genes in fission yeast. (2012)
  • 著者名/発表者名: Edwige Hiriart, Aurelia Vavasseur, Leila Touat-Todeschini, Akira Yamashita, Benoit Gilquin, Emeline Lambert, Jonathan Perot, Yuichi Shichino. Nicolas Nazaret, Cyril Boyault, Joel Lachuer, Daniel Perazza, Masayuki Yamamoto and Andre Verdel
  • 雑誌名: EMBO Journal 巻: 31 号: 10 ページ: 2296-2308
  • DOI: 10.1038/emboj.2012.105
  • 査読あり
• [雑誌論文] Meiosis-specific non-coding RNA mediates robust pairing of homologous chromosomes in meiosis (2012)
  • 著者名/発表者名: Ding D-Q, Okamasa K, Yamane M, Tsutsumi C, Haraguchi T, Yamamoto M, and Hiraoka Y
  • 雑誌名: Science 巻: 336 号: 6082 ページ: 732-736
  • DOI: 10.1126/science.1219518
  • 査読あり
• [雑誌論文] Signaling pathways for fission yeast sexual differentiation at a glance. (2012)
  • 著者名/発表者名: Yoko Otsubo
  • 雑誌名: Journal of Cell Science 巻: 125 号: 12 ページ: 2789-2793
  • DOI: 10.1242/jcs.094771
  • 査読あり
• [雑誌論文] Psk1, an AGC kinase family member in fission yeast, is directly phosphorylated and controlled by TORC1 and functions as S6 kinase. (2012)
  • 著者名/発表者名: Akio Nakashima
  • 雑誌名: Journal of Cell Science 巻: 125 号: 23 ページ: 5840-5849
  • DOI: 10.1242/jcs.111146
  • 査読あり
• [雑誌論文] The fission yeast stress-responsive MAPK pathway promotes meiosis via the phosphorylation of Pol II CTD in response to environmental and feedback cues (2011)
  • 著者名/発表者名: Y. Sukegawa, A. Yamashita and M. Yamamoto
  • 雑誌名: PLoS Genet 巻: 7 号: 12 ページ: e1002387-e1002387
  • DOI: 10.1371/journal.pgen.1002387
  • 査読あり
• [雑誌論文] A novel fission yeast mei4 mutant that allows efficient synchronization of telomere dispersal and the first meiotic division (2011)
  • 著者名/発表者名: Y. Kakui, M. Sato, K. Tanaka and M. Yamamoto
  • 雑誌名: Yeast 巻: 28 ページ: 467-479
  • 査読あり
• [雑誌論文] Pob1 ensures cylindrical cell shape by coupling two distinct rho signaling events during secretory vesicle targeting (2011)
  • 著者名/発表者名: K. Nakano, M. Toya, A. Yoneda, Y. Asami, A. Yamashita, N. Kamasawa, M. Osumi and M. Yamamoto
  • 雑誌名: Traffic 巻: 12 ページ: 726-739
  • 査読あり
• [雑誌論文] Pob1 ensures cylindrical cell shape by coupling two distinct rhosignaling events during secretory vesicle targeting (2011)
  • 著者名/発表者名: Nakano, Kentaro
  • 雑誌名: Traffic 巻: 12 ページ: 726-739
  • 査読あり
• [雑誌論文] A novel fission yeast mei4 mutant that allows efficient synchronization of telomere dispersal and the first meiotic division. (2011)
  • 著者名/発表者名: Kakui Yasutaka
  • 雑誌名: Yeast 巻: 28
  • 査読あり
• [雑誌論文] The selective elimination of messenger RNA underlies the mitosis-meiosis switch in fission yeast (2010)
  • 著者名/発表者名: M. Yamamoto
  • 雑誌名: Proc. Jpn. Acad. Ser. B Phys. Biol. Sci 巻: 86 ページ: 788-797
  • NAID: 130000442352
  • 査読あり
• [雑誌論文] Nuclear compartmentalization is abolished during fission yeast meiosis (2010)
  • 著者名/発表者名: K. Arai, M. Sato, K. Tanaka and M. Yamamoto
  • 雑誌名: Curr. Biol 巻: 20 ページ: 1913-1918
  • 査読あり
• [雑誌論文] TOR and sexual development in fission yeast (2010)
  • 著者名/発表者名: Y. Otsubo and M. Yamamoto
  • 雑誌名: The Enzymes 巻: 27 ページ: 229-250
  • 査読あり
• [雑誌論文] Importance of polyadenylation to the selective elimination of meiotic mRNAs in growing S. pombe cells (2010)
  • 著者名/発表者名: S. Yamanaka, A. Yamashita, Y. Harigaya, R. Iwata and M. Yamamoto
  • 雑誌名: EMBO J 巻: 29 ページ: 2173-2181
  • 査読あり
• [雑誌論文] Contribution of dynein light intermediate and intermediate chains to subcellular localization of the dynein-dynactin motor complex in Schizosaccharomyces pombe (2010)
  • 著者名/発表者名: I. Fujita, A. Yamashita and M. Yamamoto
  • 雑誌名: Genes Cells 巻: 15 ページ: 359-372
  • 査読あり
• [雑誌論文] Importance of polyadenylation to the selective elimination of meioticm RNAs in growing S.pombe cells. (2010)
  • 著者名/発表者名: Yamanaka Soichiro
  • 雑誌名: EMBO Journal 巻: 29 ページ: 2173-2181
  • 査読あり
• [雑誌論文] Nuclear compartmentalization is abolished during fission yeast meiosis. (2010)
  • 著者名/発表者名: Arai Kunio
  • 雑誌名: Current Biology 巻: 20 ページ: 1913-1918
  • 査読あり
• [雑誌論文] The selective elimination of messenger RNA underlies the mitosis-meiosis switch in fission yeast. (2010)
  • 著者名/発表者名: Yamamoto Masayuki
  • 雑誌名: Proc.Jpn.Acad.Ser.B Phys.Biol.Sci. 巻: 86 ページ: 788-797
  • NAID: 130000442352
  • 査読あり
• [雑誌論文] Contribution of dynein light intermediate and intermediate chains to subcellular localization of the dynein-dynactin motor complex in Schizosaccharomyces pombe. (2010)
  • 著者名/発表者名: Fujita, Ikumi
  • 雑誌名: Genes to Cells ページ: 359-372
  • 査読あり
• [雑誌論文] TOR and sexual development in fission yeaxt. (2010)
  • 著者名/発表者名: Otsubo, Yoko
  • 雑誌名: The Enzymes 27 ページ: 229-250
• [雑誌論文] Nucleocytoplasmic transport of Alp7/TACC organizes spatiotemporal microtubule formation in fission yeast. (2009)
  • 著者名/発表者名: Sato, Masamitsu
  • 雑誌名: EMBO Reports 10 ページ: 1161-1167
  • 査読あり
• [学会発表] Cuf2/Sms5 boosts the transcription of APC/C activator Fzr1/Mfr1 to terminate the meiotic division cycle. (2013)
  • 著者名/発表者名: Yuki Aoi
  • 学会等名: The Seventh International Fission Yeast Meeting
  • 発表場所: Lonon, U.K.
  • 招待講演
• [学会発表] Regulatory mechanism to activate the M-phase promoting factor during meiosis by an RNA-binding protein Spo5 in fission yeast. (2013)
  • 著者名/発表者名: Mayumi Arata
  • 学会等名: The Seventh International Fission Yeast Meeting
  • 発表場所: Lonon, U.K.
• [学会発表] Functional analysis of meiRNA, a non-coding RNA species essential for meiosis in fission yeast. (2013)
  • 著者名/発表者名: Yuichi Shichino
  • 学会等名: The Seventh International Fission Yeast Meeting
  • 発表場所: Lonon, U.K.
• [学会発表] Importance of posttranscriptional and transcriptional regulations by Spo5 and Pcr1 during fission yeast meiosis. (2013)
  • 著者名/発表者名: Naoyuki Togashi
  • 学会等名: The Seventh International Fission Yeast Meeting
  • 発表場所: Lonon, U.K.
• [学会発表] RNA degradation switches mitotic growth to meiotic development in fission yeast (2012)
  • 著者名/発表者名: M. Yamamoto
  • 学会等名: CDB meeting on "RNA Sciences in Cell and Developmental Biology II"
  • 発表場所: Kobe, Japan
  • 年月日: 2012-06-11
• [学会発表] Control of meiosis by RNA destruction in fission yeast (2012)
  • 著者名/発表者名: M. Yamamoto
  • 学会等名: Gordon Research Conference on Meiosis
  • 発表場所: New Hampshire, U.S.A.
  • 年月日: 2012-06-06
• [学会発表] Pursuing Meiosis (2011)
  • 著者名/発表者名: 山本 正幸
  • 学会等名: 第34回日本分子生物学会年会
  • 発表場所: 横浜
  • 年月日: 2011-12-15
  • 招待講演
• [学会発表] Pursuing Meiosis (2011)
  • 著者名/発表者名: 山本正幸
  • 学会等名: 第34回日本分子生物学会年会
  • 発表場所: 横浜(招待講演)
  • 年月日: 2011-12-15
• [学会発表] Regulatory network controlling the activity of Mei2, the master meiotic regulator in fission yeast (2011)
  • 著者名/発表者名: M. Yamamoto
  • 学会等名: The 6th UK-Japan Cell Cycle Workshop
  • 発表場所: Windermere, UK
  • 年月日: 2011-04-11
• [学会発表] Regulatory network controlling the activity of Mei2, the master meiotic regulator in fission yeast (2011)
  • 著者名/発表者名: Yamamoto, Masayuki
  • 学会等名: The 6th UK-Japan Cell Cycle Workshop
  • 発表場所: Windermere, UK(招待講演)
  • 年月日: 2011-04-11
• [学会発表] Regulation of meiosis-specific gene expression in fission yeast (2010)
  • 著者名/発表者名: M. Yamamoto
  • 学会等名: Gordon Research Conference on Meiosis
  • 発表場所: New Hampshire, U.S.A.
  • 年月日: 2010-06-16
• [学会発表] Regulation of meiosis-specific gene expression in fission yeast. (2010)
  • 著者名/発表者名: Yamamoto Masayuki
  • 学会等名: Gordon Research Conference on Meiosis
  • 発表場所: New Hampshire, U.S.A.
  • 年月日: 2010-06-16
• [学会発表] Molecular basis for the selective elimination of meiosis-specific mRNAs in growing cells (2009)
  • 著者名/発表者名: M. Yamamoto
  • 学会等名: The 5th International Fission Yeast Meeting
  • 発表場所: Tokyo, Japan
  • 年月日: 2009-10-26
• [学会発表] Molecular basis for the selective elimination of meiosis-specific mRNAs in growing cells. (2009)
  • 著者名/発表者名: Yamamoto, Masayuki
  • 学会等名: The 5th International Fission Yeast Meeting
  • 発表場所: Yoyogi, Tokyo, Japan
  • 年月日: 2009-10-26
• [学会発表] Molecular basis for the selective elimination of meiosis-specific mRNAs in growing fission yeast cells (2009)
  • 著者名/発表者名: M. Yamamoto
  • 学会等名: The EMBO Conference on Meiosis
  • 発表場所: Isle sur la Sorge, France
  • 年月日: 2009-09-22
• [学会発表] Molecular basis for the selective elimination of meiosis-specific mRNAs in growing fission yeast cells. (2009)
  • 著者名/発表者名: Yamamoto, Masayuki
  • 学会等名: The EMBO Conference on Meiosis
  • 発表場所: Isle sur la Sorge, France
  • 年月日: 2009-09-22
• [備考]
  • URL: http://www.biochem.s.u-tokyo.ac.jp/yamamoto-lab/J/top-f.html
• [備考]
  • URL: http://www.biochem.s.u-tokyo.ac.jp/yamamoto-lab/index.html
• [備考]
  • URL: http://www.biochem.s.u-tokyo.as.jp/yamamoto-lab/index.html