| 研究課題/領域番号 |
21H02490
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| 研究種目 |
基盤研究(B)
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| 配分区分 | 補助金 |
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| 応募区分 | 一般 |
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| 審査区分 |
小区分44020:発生生物学関連
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| 研究機関 | 熊本大学 |
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研究代表者 |
Sheng Guojun 熊本大学, 国際先端医学研究機構, 特別招聘教授 (20399439)
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| 研究分担者 |
永井 宏樹 熊本大学, 国際先端医学研究機構, リサーチ・スペシャリスト (80772508)
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| 研究期間 (年度) |
2021-04-01 – 2024-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
17,420千円 (直接経費: 13,400千円、間接経費: 4,020千円)
2023年度: 5,070千円 (直接経費: 3,900千円、間接経費: 1,170千円)
2022年度: 5,590千円 (直接経費: 4,300千円、間接経費: 1,290千円)
2021年度: 6,760千円 (直接経費: 5,200千円、間接経費: 1,560千円)
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| キーワード | EMT / chicken / hemangioblast / hematopoiesis / vasculogenesis / Embryo |
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| 研究開始時の研究の概要 |
EMT is an important morphogenetic process. Its mis-regulation is associated with cancers. EMT also plays a role in hematopoietic development and disease. In this project, we will use the avian model and investigate the role of partial EMT during primitive hematopoiesis. We will characterize partial epithelial and mesenchymal features of hemangioblasts from stages HH4-10; analyze functional involvement of two EMT transcription factors (Snai2, Zeb2); investigate cross-talk between EMT TFs and hemangioblast TFs in partial EMT regulation and promote blood/vessel specification via EMT modulation.
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| 研究実績の概要 |
In FY2022, we have performed experiments on effect of overexpression of Snai2 and Zeb2 in lineage contribution (blood, vessel, smooth muscle lineages). We have found that these EMT markers do not clearly affect lineage marker gene expression. However, they affect cell lineage segregation, likely after lineage specification. We have also investigated relationship between hemangioblast specifiers (NPAS4L and ETV2, two markers upstream of blood/vessel markers SCL/LMO2 and smooth muscle markers HAND1/2) and EMT regulators. As EMT occurs both before (during mesoderm formation) and after hemagioblast specification and at least SNAI2 is involved in both primitive streak EMT and lateral plate mesoderm EMT (subject of this project), we are in the process of sorting out epistatic relationship between SNAI2, NPAS4L, and SCL/LMO2/HAND2).
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The project is making progress as planned.
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| 今後の研究の推進方策 |
In FY2023, we will continue the project with the same direction outlined in our grant application. We will study relationship of EMT regulators (SNAI2 and ZEB2) and cell biological markers, including NCAM, a cell surface markers which we have found to correlated with hemangioblast differentiation, and VE-cadherin, a vascular specific adherens junction molecule marker endothelial type of epithelial organization. We aim to submit the results of this project for publication by the end of this fiscal year.
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