| 研究課題/領域番号 |
21J11332
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| 研究種目 |
特別研究員奨励費
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| 配分区分 | 補助金 |
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| 応募区分 | 国内 |
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| 審査区分 |
小区分37010:生体関連化学
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| 研究機関 | 東京大学 |
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研究代表者 |
HU Lingyun 東京大学, 理学系研究科, 特別研究員(DC2)
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| 研究期間 (年度) |
2021-04-28 – 2023-03-31
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| 研究課題ステータス |
完了 (2022年度)
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| 配分額 *注記 |
1,500千円 (直接経費: 1,500千円)
2022年度: 700千円 (直接経費: 700千円)
2021年度: 800千円 (直接経費: 800千円)
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| キーワード | DNA / Artificial DNA / Metal-mediated base pair / Cu(II) / DNA technology / Triazole-4-carboxylate / Artificial base pair / DNA nanotechnology |
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| 研究開始時の研究の概要 |
Metal-mediated base pairs, consisting of two ligand-type artificial nucleobases bridged with a metal ion, have been widely used for constructing metal-responsive functional DNAs. This study aims to develop novel metal-mediated base pairs capable of efficient replication by DNA polymerases. After rational design and synthesis of ligand-type nucleobases, their base paring capability in the presence of metal ions will be studied and the enzymatic recognition capability will be examined with various polymerases. The selected base pair will be applied to SELEX for deriving unique functional DNAs.
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| 研究実績の概要 |
This study aims to develop suitable metal-mediated base pairs that can be enzymatically incorporated into the DNA. During my last year’s research, I have made several progresses as listed below:
1.Designed and synthesized several novel nucleobases based on the criteria concluded from the previous research and obtained oligonucleotides containing one or more such base pairs. Apart from the ImOC, which was reported previously, the TazC is a novel candidate.
2.Thermal melting analysis of DNA duplexes containing artificial nucleobases were conducted in the presence and absence of various metal ions, in which the TazC was found to form stable TazC-Cu(II)-TazC base pair.
3.ESI-TOF MS measurements were conducted on the TazC base pair, and the formation of the Cu(II)-mediated TazC base pair was confirmed.
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| 現在までの達成度 (段落) |
令和4年度が最終年度であるため、記入しない。
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| 今後の研究の推進方策 |
令和4年度が最終年度であるため、記入しない。
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