| 研究実績の概要 |
In this year, we evaluated the barrier effects of poly-L-lysine/dextran nanofilms (PLL/D NFs) constructed via the cation-dipole interaction to maintain compartmentalized cell co-culture of NHDF and HUVEC. PLL/D NFs exhibited comparable abilities to Col-IV/LM NFs in terms of cell adhesion, proliferation, and differentiation. However, it is worth noting that PLL/D NFs maintained compartmentalized cell co-culture for over two weeks, much longer than assembled nanofilms based on ECM proteins. Furthermore, the secretion of type IV collagen was observed under the confluent HUVEC monolayer, suggesting the re-construction of natural BMs in the in-vitro cultured tissues. The efficient barrier effects of PLL/D NFs provided a powerful support for constructing organized 3D tissues in vitro.
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