| 研究課題/領域番号 |
21K03735
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| 研究種目 |
基盤研究(C)
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| 配分区分 | 基金 |
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| 応募区分 | 一般 |
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| 審査区分 |
小区分17050:地球生命科学関連
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| 研究機関 | 東京工業大学 |
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研究代表者 |
ママジャノフ イリーナ 東京工業大学, 地球生命研究所, 特任教授 (80776261)
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| 研究分担者 |
ジャー トニーズィ 東京工業大学, 地球生命研究所, 特任助教 (10800328)
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| 研究期間 (年度) |
2021-04-01 – 2022-03-31
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| 研究課題ステータス |
完了 (2021年度)
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| 配分額 *注記 |
4,030千円 (直接経費: 3,100千円、間接経費: 930千円)
2023年度: 780千円 (直接経費: 600千円、間接経費: 180千円)
2022年度: 780千円 (直接経費: 600千円、間接経費: 180千円)
2021年度: 2,470千円 (直接経費: 1,900千円、間接経費: 570千円)
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| キーワード | hyperbranched polymer / polyester / wet-dry cycle / smart material / protoenzyme / biomimetic catalysis / chemical evolution / origin of life / nanoparticle / biomimetic catalyst |
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| 研究開始時の研究の概要 |
Key question: How did enzymes emerge and evolve from prebiotic systems?
Hypothesis: Protoenzymes employed metallic and small molecule cofactors, similar to modern ones, which were scaffolded by prebotically available globular polymers, such as HBP instead of proteins and RNA.
Research methods: The project would include the study of the synthesis of HBP-NPs under prebiotic conditions and their characterization.
Importance: A successful outcome of the project would further our fundamental understanding of the origin of enzymatic catalysis, a mechanism that governs all biochemical transformations.
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| 研究実績の概要 |
We developed solid-state nuclear magnetic resonance (NMR) methods for detecting water bound and immobilized inside hyperbranched polymers. Based on the results we were able to classify the different polymers based on their water content. This property will become important at the later stages when designing catalysts for specific reactions. We optimized synthesize and analyze depsipeptide hyperbranched polymers. The polymers are synthesized by reacting a polyester matrix with amino acids. We have devised optimal amino acid mixes that allow for detectable selection without overwhelming the analysis.
We studied in more detail a lysine based hyperbranched polymer and it's catalytic ability. We found that polysine is capable of efficient catalysis of Kemp elimination.
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