| 研究課題/領域番号 |
21K06043
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| 研究種目 |
基盤研究(C)
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| 配分区分 | 基金 |
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| 応募区分 | 一般 |
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| 審査区分 |
小区分43020:構造生物化学関連
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| 研究機関 | 東京工業大学 (2022)
国立研究開発法人理化学研究所 (2021) |
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研究代表者 |
Malay Ali 東京工業大学, 物質理工学院, 研究員 (40467006)
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| 研究期間 (年度) |
2021-04-01 – 2024-03-31
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| 研究課題ステータス |
交付 (2022年度)
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| 配分額 *注記 |
4,160千円 (直接経費: 3,200千円、間接経費: 960千円)
2023年度: 260千円 (直接経費: 200千円、間接経費: 60千円)
2022年度: 390千円 (直接経費: 300千円、間接経費: 90千円)
2021年度: 3,510千円 (直接経費: 2,700千円、間接経費: 810千円)
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| キーワード | silk / biomimetic / liquid phase separation / coacervate / nanofibril / self-assembly / biopolymer / biomolecular condensate / biomimetics / phase separation / spider / biomaterial / biochemistry / silk protein |
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| 研究開始時の研究の概要 |
Spider silk is a protein biopolymer with extreme mechanical properties; however, the mechanism for silk fiber formation is not well understood. Recently, we found that spider silk undergoes self-assembly via liquid-liquid phase separation and nanoscale fibril formation in response to different chemical stimuli. In this project we will explore the sequence features of silk proteins that govern the the self-assembly process. These results will help develop methods to produce artificial spider silk fibers that mimic the hierarchical structure and mechanical properties of natural spider silk.
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| 研究実績の概要 |
This year significant progress was achieved on the work related to the Kakenhi grant. The experimental work related to the investigation on MaSp1 assembly (in isolation) has been completed, including work on the 2D protein structure as well as real-time characterization of the complete self-assembly process at the mesoscale. These results have been written up in a paper for publication.
Work on the optimization of conditions for the composite function of the different MaSp variants have been carried out, which were successful on the small-scale, however the large-scale fiber spinning and characterization has not yet been successfully completed. The analysis of the array of MaSp2 variants with variations in the repetitive domain amino acid motifs have been carried, including data collection and analysis at SPring-8 using the SEC-SAXS system, have been performed; in addition, experiments exploring the effect of mutations on reversibility/efficiency of LLPS were carried out, although these have not been finalized. The results of these works are currently being written up for publication.
Three peer-reviewed papers related to spider silk structure/function were successfully published (including 1 as co-first author), and conferences were attended.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Much progress was achieved this year, and work is still undergoing. For instance, for the study on MaSp1 (in isolation) experimental work has been completed and the manuscript has been written, but so far it has not been successfully published yet. For the other aspects, such as the study on the synergistic effects of MaSp1/2/3 spidroins, the work has not been fully completed; much of these relate to the difficulty of optimizing the experimental conditions, but it is also related to delays related to scheduling of beam-time at SPring-8, as well as to the effect of moving to a new institution (Tokyo Institute of Technology).
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| 今後の研究の推進方策 |
In the following year, I wish to pursue the experiments related to determining the effects of the different repetitive sequence motifs of MaSp proteins on the self-assembly of recombinant spider silk, as well as on the composite nature of the structure and function of spider dragline silk, until the successful publication of results in high-impact publications.
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