| 研究課題/領域番号 |
21K06111
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| 研究種目 |
基盤研究(C)
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| 配分区分 | 基金 |
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| 応募区分 | 一般 |
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| 審査区分 |
小区分43040:生物物理学関連
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| 研究機関 | 金沢大学 |
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研究代表者 |
Marchesi Arin 金沢大学, ナノ生命科学研究所, 特任助教 (80882240)
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| 研究期間 (年度) |
2021-04-01 – 2022-03-31
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| 研究課題ステータス |
中途終了 (2021年度)
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| 配分額 *注記 |
4,160千円 (直接経費: 3,200千円、間接経費: 960千円)
2023年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2022年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2021年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
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| キーワード | annexins / atomic force microscopy / membrane proteins / Annexin V / ion channel |
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| 研究開始時の研究の概要 |
The ultimate goal of the project is to visualize AnxA5 supramolecular organization,
dynamics, and function within native cellular environments.
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| 研究実績の概要 |
The aim of this project was to understand how different biophysical and biochemical parameters regulate annexin A5 supramolecular assembly and ultimately visualize annexin arrays on native membranes from isolated cells. We obtained preliminary data demonstrating that membrane fluidity is a key driver regulating annexin polycrystalline lattice formation and crystallographic configuration. The inital experimental observations were carried out on model membranes (planar artificial bilayer)by means of fluorescence imaging and high-speed atomic force microscopy. Photoswitchable compounds were also used to manipulate membrane properties and demonstrate annexin supramolecular polymorphism.
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