研究課題/領域番号 |
21K14029
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研究種目 |
若手研究
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配分区分 | 基金 |
審査区分 |
小区分17050:地球生命科学関連
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研究機関 | 東京工業大学 |
研究代表者 |
蟻 瑞欽 東京工業大学, 地球生命研究所, 研究員 (00860090)
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研究期間 (年度) |
2021-04-01 – 2024-03-31
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研究課題ステータス |
交付 (2022年度)
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配分額 *注記 |
2,860千円 (直接経費: 2,200千円、間接経費: 660千円)
2022年度: 1,040千円 (直接経費: 800千円、間接経費: 240千円)
2021年度: 1,820千円 (直接経費: 1,400千円、間接経費: 420千円)
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キーワード | amino acids / activating chemistry / peptide synthesis / RNA polymerization / solid-phase synthesis / activation chemistry / phosphorylation / polymerization |
研究開始時の研究の概要 |
Recently, we proposed a “continuous reaction networks” model, and demonstrated that a wide variety of key precursors, useful for the synthesis of RNA monomers and amino acids, are constantly formed. The remaining key question of this model is whether this continuous reaction model can further enable the subsequent step towards RNA polymers generation compatible with forming other important substrates, such as oligopeptides. Therefore, this study aims for understanding how to generate RNA monomers, amino acids, as well as their polymers in a continuous manner under a single-pot environment.
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研究実績の概要 |
The proposed cyanogen chloride induced pathway for the synthesis of RNA and peptides includes: 1. phosphorylation of nucleosides to nucleotides; 2. activation of nucleotides to their 5′-phosphorimidazoles for RNA polymerization;3. activation of amino acids to peptides and deprotection. This year, the feasibility of cyanogen chloride activated polymerization of amino acids in the solid-phase peptide synthesis has been demonstrated. We also observed that activation of nucleotides to their 5′-phosphorimidazoles for RNA polymerization and amino acids polymerization can occur in a one-pot synthesis using cyanogen chloride as a common activating agent.
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現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
This project was divided into two stages. Stage 1 is to explore cyanogen chloride activated phosphorylation of nucleosides to nucleotides, and stage 2 is to assess cyanogen chloride (ClCN) activated polymerization of amino acids to peptides. This project was scheduled to be finished in two years. In the original plan, stage 1 would be finished last year. Since I already had some primary result of stage 2, so I changed the sequence of the project plan and continued to explore the feasibility of stage 2 and finished the goals of stage 2.
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今後の研究の推進方策 |
All the experiments in the stage 2 have been finished. I am writing the paper now to summary the whole results in the stage 2. I will continue to explore the possibility of stage 1 this year.
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