研究課題/領域番号 |
21K14169
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研究種目 |
若手研究
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配分区分 | 基金 |
審査区分 |
小区分21030:計測工学関連
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研究機関 | 岡山大学 |
研究代表者 |
WANG JIN 岡山大学, ヘルスシステム統合科学学域, 助教 (10870975)
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研究期間 (年度) |
2021-04-01 – 2024-03-31
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研究課題ステータス |
交付 (2022年度)
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配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2023年度: 1,170千円 (直接経費: 900千円、間接経費: 270千円)
2022年度: 1,560千円 (直接経費: 1,200千円、間接経費: 360千円)
2021年度: 1,950千円 (直接経費: 1,500千円、間接経費: 450千円)
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キーワード | peptide / computational design / small molecules / odorant binding protein / docking / organic receptors / 計算分子設計 / ペプチド / プラズモニック / がんやCOVID-19のVOCsバイオマーカー |
研究開始時の研究の概要 |
Peptide as a promising molecular recognition element has gained intense interest for developing novel sensing devices. In this study, rational design of peptide based plasmonic biosensor for small molecules (volatile organic compounds biomarkers from cancer/disease and COVID-19) will be developed.
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研究実績の概要 |
A series of peptides, derived from odorant-binding proteins (OBPs), were strategically designed to recognize VOCs associated with SARS-CoV-2. Ethanol, nonanal, benzaldehyde, acetic acid, and acetone were chosen as representative VOCs found in exhaled breath during COVID-19 infection. Computational docking and prediction tools were employed to characterize and analyze the OBP peptides. Various factors, such as docking models, binding affinity, sequence specificity, and structural folding, were examined.
Additionally, bioreceptors such as DNA aptamers and organic receptors like APTES have been confirmed and utilized for highly sensitive detection of small molecules, such as cortisol and aromatic compounds. The interaction mechanisms were demonstrated using computational docking approaches.
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現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Although sensor recognition has been successfully developed and demonstrated using various methods, the next step should focus on the development of a plasmonic sensor.
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今後の研究の推進方策 |
Therefore, the next step in our research will focus on developing a peptide-gold nanoparticles, PDMS-based wearable sensor for VOCs detection. This innovative approach will capitalize on the localized surface plasmon resonance (LSPR) phenomenon induced by gold nanoparticles, which is known for its enhanced sensitivity and selectivity. By incorporating the peptide-based recognition elements onto the gold nanoparticles, we aim to create a highly responsive and reliable wearable sensor for detecting VOCs in real-time. This cutting-edge technology has the potential to revolutionize VOCs sensing applications, from environmental monitoring to medical diagnostics and beyond.
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