| 研究課題/領域番号 |
21K14804
|
|---|
| 研究種目 |
若手研究
|
| 配分区分 | 基金 |
|---|
| 審査区分 |
小区分38050:食品科学関連
|
|---|
| 研究機関 | 広島大学 |
|---|
研究代表者 |
KUMRUNGSEE THANUTCHAPORN (カムランシー タナッチャポーン) 広島大学, 統合生命科学研究科(生), 准教授 (90781849)
|
|---|
| 研究期間 (年度) |
2021-04-01 – 2024-03-31
|
| 研究課題ステータス |
交付 (2022年度)
|
| 配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2023年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円)
2022年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円)
2021年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
|
|---|
| キーワード | Carnosine / Muscle regeneration / Skeletal muscle / Vitamin B6 / sarcopenia / Satellite cells / Twist2 |
|---|
| 研究開始時の研究の概要 |
For decades, carnosine has been believed to improve exercise performance and prevent sarcopenia, but its exact molecular mechanisms remain unknown. This study aims to clarify if carnosine has any effects on muscle satellite cells (SCs, Pax7+ cells) and recently identified muscle progenitor cells (PCs, Tw2+ cells), essential for skeletal muscle regeneration and maintaining muscle mass, respectively. Moreover, this study will determine how carnosine plays a role in muscle regeneration and muscle energy metabolism.
|
| 研究実績の概要 |
In this year, we further conducted experiments to determine if a carnosine regulator, vitamin B6, has any effects on muscle regeneration. We found that vitamin B6 deficient (1 mg PN* HCl/kg diet) and supplemented (35 mg/kg diet) diets significantly decreased carnosine levels in skeletal muscles, as compared to vitamin B6 recommended level diet (7 mg/kg diet). Next, we found that vitamin B6 deficiency (1 mg/kg diet) impaired muscle regeneration, reflecting by lower regenerating muscle mass and smaller myofiber size than the recommended vitamin B6 level (7 mg/kg diet) diet. Surprisingly, a high dose of vitamin B6 (35 mg/kg diet) also worsened muscle regeneration, which was against our expectation. Taken together the results suggest that the proper intake levels of vitamin B6 and higher levels of muscle carnosine are important to ensure the proper process of muscle regeneration. PN:Pyridoxine Hydrochloride
|
| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
More research collaborators have joined my group so that more work can be done. So, everything has gone smoothly.
|
| 今後の研究の推進方策 |
In FY 2023, effects of vitamin B6 on satellite cell activity will be examine by applying a single myofiber technique. In addition, effects of vitamin B6 on muscle hypertrophy in aging mice will be determined.
|
