| 研究課題/領域番号 |
21K14818
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分38050:食品科学関連
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| 研究機関 | 広島大学 |
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研究代表者 |
NGUYEN VANQUAN 広島大学, 先進理工系科学研究科(国), 研究員 (90883033)
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| 研究期間 (年度) |
2021-04-01 – 2025-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
4,550千円 (直接経費: 3,500千円、間接経費: 1,050千円)
2024年度: 910千円 (直接経費: 700千円、間接経費: 210千円)
2023年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2022年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
2021年度: 910千円 (直接経費: 700千円、間接経費: 210千円)
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| キーワード | momilactones / anti-blood cancer / exogenous application / bioaccessibility / in vitro digestion / cytotoxicity / blood cancer / momilactone / anticancer / rice / phytocompounds / bioavailability |
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| 研究開始時の研究の概要 |
This project will be conducted from April, 2021 to March, 2025 in order to elucidate the effect of MA and MB and natural anticancer compounds derived from rice on various cancers; and to discover the optimal ratios of the natural compounds that can be efficiently applied in therapeutics.
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| 研究実績の概要 |
- The anti-blood cancer capacity of momilactones A (MA) and B (MB) was confirmed and validated by MTT assay on other cancer cell lines including OUN-1, U937, THP-1, Jurkat, Molt-4, and YT. The IC50 values ranged from 0.5 - 5.0 ug/mL (safe for normal human cells). MB exhibited stronger activity than MA. Additionally, the cytotoxicity of MA and MB on pancreatic and lung cancer cell lines has been evaluated. The results are being analyzed and prepared for a manuscript.
- The preliminary study on exogenous application for improvement of MA and MB contents in rice and rice products was conducted. Accordingly, Koshihikari brown rice germinated under 75 mM salinity (NaCl) for 4 days impoved MA and MB contents by 3 and 2 times, respectively compared to control (0 mM, 4 days-germination). Besides, other isolated compounds tricin and p-coumaric also increased the contents 3 and 1.5 times, respectively at the same treatment. The results were published on Foods: https://doi.org/10.3390/foods12132501.
- Remarkably, we have successfully simplified the extraction process to obtain extracts rich in MA and MB from rice bran by applying the principle of freezing and thawing of raw materials combined with liquid-solid extraction using only one type of organic solvent. The results are being synthesized to prepare a manuscript.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
- We have perfected the extraction and isolation process of potential anticancer compounds from rice husk, including MA, MB, p-coumaric acid, and tricin. Additionally, we have simplified the extraction process to obtain higher concentrations of MA and MB compared to traditional methods.
- The anti-blood cancer activity of MA and MB has been validated through both cytotoxicity experiments and mechanism of action studies. Preliminary evaluations of their activities against pancreatic and lung cancers are underway.
- The experiments on momilactone combinations with synthetic drugs and other isolated compounds have not been completed due to the lengthy process of synthesis and analysis of end products. These experiments are currently in the process of optimizing procedures and will be carried out in the next phase of the project. Alongside comprehensive collaboration with Aichi Medical University, Kanazawa University, and Toyama University, we anticipate achieving significant results in the final stage of the project.
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| 今後の研究の推進方策 |
- Study on absorbability of MA and MB through digestive tract of mice (in vivo digestion).
- To complete evaluating the anticancer activity of combinations of momilactones, tricin, p-coumaric acid, and other synthetic anticancer drugs.
- To evaluate anticancer ability of momilactone’s derivatives created by various hydrolysis and derivatization methods.
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