| 配分額 *注記 |
4,550千円 (直接経費: 3,500千円、間接経費: 1,050千円)
2022年度: 2,470千円 (直接経費: 1,900千円、間接経費: 570千円)
2021年度: 2,080千円 (直接経費: 1,600千円、間接経費: 480千円)
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| 研究開始時の研究の概要 |
Reproductive aging implies a sharp decline in female fertility and an increase in pregnancy complications with
maternal age. The major characteristics of the reproductive aging are low birth rate/embryonic malformation caused by defective decidualization at early pregnancy, and the greater risk of operative delivery due to prolong gestational period at late pregnancy. In this study, we compare the difference of hormone levels and the gene expression profiles between young and aged pregnant female mice to develop a novel strategy to get healthy babies in aged women similar to the young.
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| 研究実績の概要 |
We investigate the reproductive aging process in mice and its implications for pregnancy complications. We have observed that aged pregnant mice experience difficulties during pregnancy, including prolonged labor and increased fetal loss, due to insufficient progesterone withdrawal in late pregnancy. This withdrawal is essential for triggering the onset of labor by activating genes involved in myometrial contraction. Through analyzing the gene expression profiles of myometrial tissue from young and aged pregnant mice, we have identified differences in genetic and epigenetic markers associated with labor onset. Additionally, we have discovered a novel target gene related to labor initiation and duration, TCF23, a Class II Basic Helix-Loop-Helix protein, showing its involvement in obstructed labor in mice. in 2023. I finalized the analysis of Tcf23 knockout mice, we observed poor responsiveness of the myometrium to remodeling and contraction. RNA-seq analysis of knockout myometrium revealed a downregulation of ECM-related genes. Additionally, we identified molecules that bind to TCF23 and control myometrial function, along with downstream targets.
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