| 研究課題/領域番号 |
21K15070
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分43050:ゲノム生物学関連
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| 研究機関 | 沖縄科学技術大学院大学 |
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研究代表者 |
枡鏡 優美子 沖縄科学技術大学院大学, 進化・細胞・共生の生物学ユニット, 技術員 (10839848)
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| 研究期間 (年度) |
2021-04-01 – 2024-03-31
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| 研究課題ステータス |
交付 (2022年度)
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| 配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2023年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円)
2022年度: 1,820千円 (直接経費: 1,400千円、間接経費: 420千円)
2021年度: 1,170千円 (直接経費: 900千円、間接経費: 270千円)
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| キーワード | Comparative genomics / Free-living bacteria / Symbiotic bacteria / Evolutional transition / Genome reduction / Multi-omics / Minimal cell / Free living bacteria / Metabolomics / Proteomics / Evolution / Mycoplasma / Endosymbiotic bacteria |
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| 研究開始時の研究の概要 |
I investigate evolution during the transition to minimal cells in mycoplasmas and insect endosymbionts using multi-omics and microscopic approaches to directly show the effects of gene presence/absence.
This study will fill a major gap in our understanding of the functioning of minimal cells.
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| 研究実績の概要 |
This research project aims to study the evolutionary transition from the simplest self-replicating bacteria to highly host-dependent symbiotic bacteria, along the path of free-living bacteria to eventually organelles. To better understand how free-living bacteria become host-dependent, the research focuses on comparative genomics on the details what kind of genes are lost or acquired. Multi-omics and microscopic approaches are combined to reveal a functional comparison of two distinct bacteria.
Detailed gene comparisons are currently performed on simplest self-replicating Mycoplasma spp. and highly host-dependent insect symbiotic bacteria Tremblaya using several different bioinformatics tools.
Multiple different electron microscopy methods are applied and tested to reveal ultrastructural morphological differences on these two distinct bacteria in their host cell systems. Metabolomics as functional comparisons are currently in progress as collaborative project with several different research departments at the University of Melbourne.
New genomic project relates to the proposal has been started as there is new interesting Spiroplasma species, same genus as Mycoplasma spp., found in the coral host. This new project has potential to answer the evolutional transition of free-living to symbiotic bacteria.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
This research project is progressing rather smoothly in 2022 as all proposed experiments and approaches are currently in progress and collaborative work has been started and progressing.
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| 今後の研究の推進方策 |
Complete comparative genomics on two distinct bacteria using multiple bioinformatics tools. Images on electron microscopy to investigate morphological differences in host cell could be improved in 2023. Collaborative work on mycoplasma infection system in cultured cell for the metabolomics and microscopy observation will be progressed in 2023.
Because we found interesting relationship of Spiroplasma spp., same genus Mollicutes as Mycoplasmas, and coral host, I expand genomics analysis on this project. This new project also has potential to shed the light on evolutional transition of free-living bacteria to symbiotic bacteria as I proposed in the research proposal.
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