| 研究課題/領域番号 |
21K15070
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分43050:ゲノム生物学関連
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| 研究機関 | 沖縄科学技術大学院大学 |
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研究代表者 |
枡鏡 優美子 沖縄科学技術大学院大学, 進化・細胞・共生の生物学ユニット, 技術員 (10839848)
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| 研究期間 (年度) |
2021-04-01 – 2025-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2023年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円)
2022年度: 1,820千円 (直接経費: 1,400千円、間接経費: 420千円)
2021年度: 1,170千円 (直接経費: 900千円、間接経費: 270千円)
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| キーワード | Comparative genomics / Free-living bacteria / Symbiotic bacteria / Evolutional transition / Genome reduction / Multi-omics / Minimal cell / Free living bacteria / Metabolomics / Proteomics / Evolution / Mycoplasma / Endosymbiotic bacteria |
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| 研究開始時の研究の概要 |
I investigate evolution during the transition to minimal cells in mycoplasmas and insect endosymbionts using multi-omics and microscopic approaches to directly show the effects of gene presence/absence.
This study will fill a major gap in our understanding of the functioning of minimal cells.
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| 研究実績の概要 |
This research project aims to study the evolutionary transition from the simplest self-replicating bacteria to highly host-dependent symbiotic bacteria, along the path of free-living bacteria to eventually organelles. To better understand how free-living bacteria become host-dependent, the research focuses on comparative genomics on the details what kind of genes are lost or acquired.
Multi-omics and microscopic approaches are performed to reveal the functional comparison of two distinct bacteria.
Detailed gene comparisons are currently in progress to compare simplest self-replicating Mycoplasma spp. and highly host-dependent insect symbiotic bacteria Tremblaya using several different bioinformatics tools.
Multiple different electron microscopy methods are applied and tested to reveal ultrastructural morphological differences on these two distinct bacteria in their host cell systems. The analysis on electron microscopy images are in progress.
The metabolomic data analysis are also currently in progress as collaborative project with the University of Melbourne.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
4: 遅れている
理由
The research project is in delay because I did not have time to visit collaboration laboratories to finish off the metabolomics work in 2023.
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| 今後の研究の推進方策 |
Electron microscopy images to investigate morphological differences in host cells were completed in 2023. In 2024, I will focus on the image analysis and finish up the imaging part of this project. Some metabolomics trials and data analyses were done by 2023. In 2024, I plan to conduct some more experiments for confirming the trial studies by visiting collaborator laboratories at the University of Melbourne.
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