| 研究課題/領域番号 |
21K15088
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分44010:細胞生物学関連
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| 研究機関 | 広島大学 |
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研究代表者 |
IJAZ FARYAL 広島大学, 医系科学研究科(医), 助教 (80845595)
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| 研究期間 (年度) |
2021-04-01 – 2024-03-31
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| 研究課題ステータス |
交付 (2022年度)
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| 配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2023年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
2022年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円)
2021年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円)
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| キーワード | Primary Cilium / Ciliopathies / primary cilium / Extracellular vesicles / Extracellular Vesicles |
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| 研究開始時の研究の概要 |
Primary cilium have recently been shown to be a source of new type of extracellular vesicle (pcEVs).This study aims to identify the molecules and pathways involved in the uptake of pcEVs which will provide insights into the regulation of pcEVs uptake in physiological and pathological conditions.
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| 研究実績の概要 |
Cell-intrinsic mechanisms of ciliopathies have been well studied. In contrast, cell-extrinsic factors that underlie ciliopathies have barely been explored.
We investigated the effects of extracellular bioactive factors derived from wildtype NIH/3T3 cells culture supernatant on the migration/ proliferation of primary cilium-deficient NIH/3T3-Kif3a-KO cells after wounding. The factors derived from wildtype NIH/3T3 cells culture supernatant increased the rate of cell migration/proliferation in target cells as compared to the factors derived from primary cilium-deficient cells culture supernatant from NIH/3T3-Kif3a-KO cells and NIH/3T3-Dync2h1-KO cells. Furthermore, by omics analysis of the supernatants, we found a molecule that showed a decline in the culture supernatant of primary cilium-deficient cells. These findings suggest that fibroblasts regulate cellular migration/proliferation process in primary cilium-deficient target cells via cell-extrinsic regulatory mechanisms in a primary cilium-dependent manner.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The project is rather going smoothly because we identified some of the factors involved in cell-extrinsic regulation of ciliopathies.
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| 今後の研究の推進方策 |
Task1. Evaluation of secretion profile: Confirmation of biofactors and signaling pathways.
Task2. Generate invitro models of ciliopathies using human cell lines.
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