| 研究課題/領域番号 |
21K15203
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分46030:神経機能学関連
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| 研究機関 | 東京大学 |
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研究代表者 |
UCAR HASAN 東京大学, ニューロインテリジェンス国際研究機構, 特任助教 (50748423)
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| 研究期間 (年度) |
2021-04-01 – 2024-03-31
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| 研究課題ステータス |
中途終了 (2022年度)
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| 配分額 *注記 |
4,550千円 (直接経費: 3,500千円、間接経費: 1,050千円)
2025年度: 910千円 (直接経費: 700千円、間接経費: 210千円)
2024年度: 1,040千円 (直接経費: 800千円、間接経費: 240千円)
2023年度: 910千円 (直接経費: 700千円、間接経費: 210千円)
2022年度: 910千円 (直接経費: 700千円、間接経費: 210千円)
2021年度: 780千円 (直接経費: 600千円、間接経費: 180千円)
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| キーワード | Presynaptic terminal / mechanical stimulation / mechanobiology / PREST / Release probabilty / Optogenetics / iGluSnFr / FRET / Spine enlargement / Mechanosensation / Synaptic plasticity / SNARE assembly / Glutamate release |
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| 研究開始時の研究の概要 |
Using 2-photon and STED-microscopy I will study the molecular mechanisms of our novel finding of the pressure sensation and transduction (PREST) mechanism in the presynaptic terminal in the brain.
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| 研究実績の概要 |
We announced mechanical transmission and PREST in CNS in 2021 (Ucar et al., Nature, 2021).
I am studying the underlying mechanisms of PREST and we published a review article to underline its physiological importance for brain function and memory.
Recently I found that mechanical stimulation activates intracellular signaling pathways which lead to synaptic vesicle regulation during PREST. Here, synaptic vesicle clusters are mobilized upon mechanical stimulation and this is estimated to be regulated by a kinase pathway that I am working on.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
I was able to conlude that presynaptic terminal responds to mechanical stimulation and made a publication in Nature (Ucar et al. Nature 2021) and described PREST (pressure sensation and transduction) mechanism. Now I am studying underlying mechanisms for which I have promising data.
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| 今後の研究の推進方策 |
Now I am studying underlying mechanisms of PREST for which I have promising data. I will continue testing new molecular probes to understand the involvement of molecules for the regulation and function of PREST and their regulation by the intracellular kinases by using two-photon microscopy.
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