| 研究課題/領域番号 |
21K15214
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分46030:神経機能学関連
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| 研究機関 | 国立研究開発法人理化学研究所 |
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研究代表者 |
Sekulic Vladislav 国立研究開発法人理化学研究所, 脳神経科学研究センター, 特別研究員 (60865492)
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| 研究期間 (年度) |
2021-04-01 – 2024-03-31
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| 研究課題ステータス |
交付 (2022年度)
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| 配分額 *注記 |
2,600千円 (直接経費: 2,000千円、間接経費: 600千円)
2023年度: 780千円 (直接経費: 600千円、間接経費: 180千円)
2022年度: 780千円 (直接経費: 600千円、間接経費: 180千円)
2021年度: 1,040千円 (直接経費: 800千円、間接経費: 240千円)
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| キーワード | hippocampus / memory / engram / interneuron / calcium imaging / physiology / memory encoding / electrophysiology / imaging / microcircuits |
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| 研究開始時の研究の概要 |
This research aims to determine how region-specific differences in memory formation within the hippocampus are governed by differences in inhibitory interneuron function. This work will be critical in determining how manipulations of interneurons can help precisely modulate memory acquisition.
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| 研究実績の概要 |
As reported in the FY2021 F-7-1 year end report, the main dataset of calcium recordings were completed more rapidly than expected, allowing time to expand the dataset. This was preformed in FY2022. I recorded pilot mice with dual imaging of immediate early gene (memory labelling) neurons as well as dynamic activity of all neurons to verify the system, and also performed an additional dataset of recordings of mice to verify the mechanism of action of the previous results. In particular, I recorded directly from the cells implicated in my earlier results using fluorescence indicators in live behaving mice to show that the effect of the chemogenic drug to shut down or activate those neurons actually worked as expected. This dataset can also be analyzed for additional insights as to how the memory is enhanced or disrupted in the behavioral tasks performed.
I furthermore performed significant data analysis of the previous main dataset and developed a novel algorithm for detecting “place field” information from the recorded neurons in an open field environment using statistical measures and machine learning tools like varational Bayesian Gaussian mixture models.
I was also able to complete a new dataset on performing tracing studies on the brain regions that provide inputs to the part of hippocampus implicated in this study, to show that the manipulation performed earlier results in changes in IEG-mediated synaptic contacts consistent with the pattern of manipulation performed, to verify the long-term effects of the memory changes.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
As outlined in the Summary of Research Achievements section above, the progress in FY2022, building on the success of the progress in FY2021, was able to significantly advance the state of the research beyond the initially planned calcium imaging dataset and is now also pursuing several additional followup avenues of investigation to provide a complete picture of the implications of the impact of the research questions being examined.
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| 今後の研究の推進方策 |
During the last few months of work on this project, the dataset analysis will be the primary focus and promoting the research at local and international meetings. I plan on presenting the research at the annual Japanese Neuroscience Society in Sendai in August 2023. I will also present the research at the Society of Neuroscience international meeting in Washington, D.C. in November 2023.
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