研究課題/領域番号 |
21K15577
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研究種目 |
若手研究
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配分区分 | 基金 |
審査区分 |
小区分50020:腫瘍診断および治療学関連
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研究機関 | 東京理科大学 |
研究代表者 |
ドアン テイキンユン 東京理科大学, 研究推進機構生命医科学研究所, 助教 (70838870)
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研究期間 (年度) |
2021-04-01 – 2024-03-31
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研究課題ステータス |
交付 (2022年度)
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配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2023年度: 520千円 (直接経費: 400千円、間接経費: 120千円)
2022年度: 2,080千円 (直接経費: 1,600千円、間接経費: 480千円)
2021年度: 2,080千円 (直接経費: 1,600千円、間接経費: 480千円)
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キーワード | OTN-NIR / MRI / Herceptin / multimodal imaging / breast cancer / herceptin / Her 2 / Breast cancer / Bioimaging / Imaging probe |
研究開始時の研究の概要 |
This project develops a bimodal OTN-NIR and MR imaging probe for targeting breast cancer. MRI can navigate and localize cancer tumors in the body. OTN-NIR can provide volumetric images of tumors with high sensitivity and resolution. This probe is promising for using in surgical aid imaging.
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研究実績の概要 |
In this fiscal year, the developed bimodal magnetic resonance imaging (MRI)/ and near-infrared fluorescence probe conjugating to Herceptin (~ 40 nm) for HER2 breast cancer targeting. The probe composed of PLGA-b-PEG micelles encapsulating IR-1061 in the core and gadolinium-tetraazacyclododecanetetraacetic acid (Gd-DOTA) conjugated at the border of PLGA/PEG. Herceptin was then conjugated through their amine residues via EDC/NHS chemistry. The study aims to assess the performance of the designed bimodal imaging probe for detecting of breast cancers and determine the optimal conditions in vitro in order to assess the detection limit of the probe, we evaluated the specific binding activities of probe concentrations.
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現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
We could developed the imaging probe for HER2 (+) breast cancer targeting. The important question is if the presence of Herceptin have any negative effect on the imaging properties. Therefore, we promoted for the screening the best conditions for detecting of breast cancers and determine the optimal conditions in vitro, in which the authors focus on the influence of carboxyl groups and their effects on probe behaviors. In order to assess the detection limit of the probe, we evaluated the specific binding activities of probe concentrations. We found the best conditions for the probe detecting antigen. The optimal condition allowed the probe maintaining its OTN-NIR/MRI properties while performing the detection of the HER-2 antigen.
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今後の研究の推進方策 |
The results are going to be reported at several international conferences. Besides, based on the results obtained from the experiment, papers were prepared and will be submitted.
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