新たな腎臓膜輸送体制御法の開発

• 研究課題/領域番号: 22249032
• 研究種目: 基盤研究(A)
• 配分区分: 補助金
• 応募区分: 一般
• 研究分野: 腎臓内科学
• 研究機関: 東京医科歯科大学
• 研究代表者: 佐々木 成 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (60170677)
• 研究期間 (年度): 2010-04-01 – 2014-03-31
• 研究課題ステータス: 完了 (2013年度)
• 配分額: 47,190千円 (直接経費: 36,300千円、間接経費: 10,890千円); 2013年度: 11,440千円 (直接経費: 8,800千円、間接経費: 2,640千円); 2012年度: 11,440千円 (直接経費: 8,800千円、間接経費: 2,640千円); 2011年度: 11,440千円 (直接経費: 8,800千円、間接経費: 2,640千円); 2010年度: 12,870千円 (直接経費: 9,900千円、間接経費: 2,970千円)
• キーワード: 水・電解質代謝学 / 塩分感受性高血圧 / 遺伝子改変マウス / アクアポリン / 膜輸送体 / 腎臓 / ケミカルライブラリー / 輸送体 / リン酸化 / 水チャネル / 高血圧 / AQP2 / WNKキナーゼ / アンギオテンシンII / SPAK

研究概要

対象とする輸送体分子（AQP水チャネル、CLCクロライドチャネル、SLC12A輸送体）のさらなる制御機構の解明と病態への関与を明らかにし、それに基づいた新たな膜輸送体制御機構を確立することを目的として本研究は開始された。その結果、AQP研究においては近位尿細管小胞体に存在するAQP11の欠損が嚢胞腎原因遺伝子PC-1の糖化と細胞膜局在を障害することで嚢胞腎を引き起こすという新たな分子機構を解明し、CLC研究においては変異バーチンの小胞体係留を軽減する薬剤を同定し、WNK研究ではWNKキナーゼ系阻害薬の単離に成功した。

研究成果

• [雑誌論文] Aberrant glycosylation and localization of polycystin-1 cause polycystic kidney in AQP11 knockout mice. (2014)
  • 著者名/発表者名: Inoue Y, Sohara E, Kobayashi K, Chiga M, Rai T, Ishibashi K, Horie S, Xuefeng Su, Jing Zhou, Sasaki S, Uchida S.
  • 雑誌名: J. Am. Soc. Nephrol 巻: in press
  • 査読あり
• [雑誌論文] Immunolocalization and translocation of aquaporin-5 water channel in sweat glands (2013)
  • 著者名/発表者名: Inoue R, Sohara E, Rai T, Satoh T, Yokozeki H, Sasaki S, Uchida S
  • 雑誌名: J Dermatol Sci 巻: 70 ページ: 26-33
  • 査読あり
• [雑誌論文] Development of enzyme-linked immunosorbent assays for urinary thiazide-sensitive Na-Cl cotransporter (NCC) measurement (2013)
  • 著者名/発表者名: Isobe K, Mori T, Asano T, Kawaguchi H, Nonoyama S, Kumagai N, Kamada F, Morimoto T, Hayashi M, Sohara E, Rai T, Sasaki S, Uchida S
  • 雑誌名: Am J Physiol Renal Physiol 巻: 305
  • 査読あり
• [雑誌論文] Chemical library screening for WNK signalling inhibitors using fluorescence correlation spectroscopy (2013)
  • 著者名/発表者名: Mori T, Kikuchi E, Watanabe Y, Fujii S, Ishigami-Yuasa M, Kagechika H, Sohara E, Rai T, Sasaki S, Uchida S
  • 雑誌名: Biochem J 巻: 455 ページ: 339-345
  • 査読あり
• [雑誌論文] Decrease of WNK4 ubiquitination by disease-causing mutations of KLHL3 through different molecular mechanisms (2013)
  • 著者名/発表者名: Mori Y, Wakabayashi M, Mori T, Araki Y, Sohara E, Rai T, Sasaki S, Uchida S
  • 雑誌名: Biochem Biophys Res Commun 巻: 439 ページ: 30-34
  • 査読あり
• [雑誌論文] Treatment with 17-allylamino-17-demethoxygeldanamycin ameliorated symptoms of Bartter syndrome type caused by mutated Bsnd in mice (2013)
  • 著者名/発表者名: Nomura N, Kamiya K, Ikeda K, Yui N, Chiga M, Sohara E, Rai T, Sasaki S, Uchida S
  • 雑誌名: Biochem Biophys Res Commun 巻: 441 ページ: 544-549
  • 査読あり
• [雑誌論文] Hereditary nephrogenic diabetes insipidus in Japanese patients : analysis of 78 families and report of 22 new mutations in AVPR2 and AQP2 (2013)
  • 著者名/発表者名: Sasaki S, Chiga M, Kikuchi E, Rai T, Uchida S
  • 雑誌名: Clin Exp Nephrol 巻: 17 ページ: 338-344
  • NAID: 10031184239
  • 査読あり
• [雑誌論文] KLHL2 interacts with and ubiquitinates WNK kinases (2013)
  • 著者名/発表者名: Takahashi D, Mori T, Wakabayashi M, Mori Y, Susa K, Zeniya M, Sohara E, Rai T, Sasaki S, Uchida S
  • 雑誌名: Biochem Biophys Res Commun 巻: 437 ページ: 457-462
  • 査読あり
• [雑誌論文] Impaired KLHL3-mediated ubiquitination of WNK4 causes human hypertension (2013)
  • 著者名/発表者名: Wakabayashi M, Mori T, Isobe K, Sohara E, Susa K, Araki Y, Chiga M, Kikuchi E, Nomura N, Mori Y, Matsuo H, Murata T, Nomura S, Asano T, Kawaguchi H, Nonoyama S, Rai T, Sasaki S, Uchida S
  • 雑誌名: Cell Rep 巻: 3 ページ: 858-868
  • 査読あり
• [雑誌論文] Dietary salt intake regulates WNK3-SPAK-NKCC1 phosphorylation cascade in mouse aorta through angiotensin II (2013)
  • 著者名/発表者名: Zeniya M, Sohara E, Kita S, Iwamoto T, Susa K, Mori T, Oi K, Chiga M, Takahashi D, Yang SS, Lin SH, Rai T, Sasaki S, Uchida S
  • 雑誌名: Hypertension 巻: 62 ページ: 872-878
  • 査読あり
• [雑誌論文] Hereditary nephrogenic diabetes insipidus in Japanese patients: analysis of 78 families and report of 22 new mutations in AVPR2 and AQP2. (2013)
  • 著者名/発表者名: Sasaki S, Chiga M, Kikuchi E, Rai T, Uchida S.
  • 雑誌名: Clin Exp Nephrol. 巻: 3 号: 3 ページ: 338-44
  • DOI: 10.1007/s10157-012-0726-z
  • NAID: 10031184239
  • 査読あり
• [雑誌論文] Chemical library screening for WNK signalling inhibitors using fluorescence correlation spectroscopy. (2013)
  • 著者名/発表者名: Mori, T.; Kikuchi, E.; Watanabe, Y.; Fujii, S.; Ishigami-Yuasa, M.; Kagechika, H.; Sohara, E.; Rai, T.; Sasaki, S.; Uchida, S.
  • 雑誌名: Biochem. J. 巻: 455 号: 3 ページ: 339-345
  • DOI: 10.1042/bj20130597
  • 査読あり
• [雑誌論文] Immunolocalization and translocation of aquaporin-5 water channel in sweat glands. (2013)
  • 著者名/発表者名: Inoue R, Sohara E, Rai T, Satoh T, Yokozeki H, Sasaki S, Uchida S.
  • 雑誌名: J Dermatol Sci. 巻: 70 号: 1 ページ: 26-33
  • DOI: 10.1016/j.jdermsci.2013.01.013
  • 査読あり
• [雑誌論文] Treatment with 17-allylamino-17-demethoxygeldanamycin ameliorated symptoms of Bartter syndrome type caused by mutated Bsnd in mice. (2013)
  • 著者名/発表者名: Nomura N, Kamiya K, Ikeda K, Yui N, Chiga M, Sohara E, Rai T, Sasaki S, Uchida S.
  • 雑誌名: Biochem Biophys Res Commun. 巻: 441 号: 3 ページ: 544-49
  • DOI: 10.1016/j.bbrc.2013.10.129
  • 査読あり
• [雑誌論文] The impact of hyporesponsiveness to erythropoietin-stimulating agents on time-dependent mortality risk among CKD stage 5D patients: a single-center cohort study. (2013)
  • 著者名/発表者名: Ishigami J
  • 雑誌名: Clin. Exp. Nephrol. 巻: 17 号: 1 ページ: 106-114
  • DOI: 10.1007/s10157-012-0659-6
  • 査読あり
• [雑誌論文] Characterization of AQP-2 gene mutation (R254Q) in a family with dominant nephrogenic DI.. (2013)
  • 著者名/発表者名: Shida Y
  • 雑誌名: Pediatr Int. 巻: 55 号: 1 ページ: 105-107
  • DOI: 10.1111/j.1442-200x.2012.03614.x
  • 査読あり
• [雑誌論文] Impaired KLHL3-mediated ubiquitination of WNK4 causes human hypertension. (2013)
  • 著者名/発表者名: Wakabayashi M
  • 雑誌名: Cell Rep. 巻: 28 号: 3 ページ: 858-868
  • DOI: 10.1016/j.celrep.2013.02.024
  • 査読あり
• [雑誌論文] Phosphorylation of Na-Cl cotransporter by OSR1 and SPAK kinases regulates its ubiquitination (2012)
  • 著者名/発表者名: Hossain Khan MZ, Sohara E, Ohta A, Chiga M, Inoue Y, Isobe K, Wakabayashi M, Oi K, Rai T, Sasaki S, Uchida S
  • 雑誌名: Biochem. Biophys. Res. Commun 巻: 425 ページ: 456-461
  • 査読あり
• [雑誌論文] Phosphatidylinositol 3-kinase/Akt signaling pathway activates the WNK-OSR1/SPAK-NCC phosphorylation cascade in hyperinsulinemic db/db mice (2012)
  • 著者名/発表者名: Nishida H, Sohara E, Nomura N, Chiga M, Alessi DR, Rai T, Sasaki S, Uchida S
  • 雑誌名: Hypertension 巻: 60 ページ: 981-990
  • 査読あり
• [雑誌論文] A minor role of WNK3 in regulating phosphorylation of renal NKCC2 and NCC co-transporters in vivo (2012)
  • 著者名/発表者名: Oi K, Sohara E, Rai T, Misawa M, Chiga M, Alessi DR, Sasaki S, Uchida S
  • 雑誌名: Biol.Open 巻: 1 ページ: 120-127
  • 査読あり
• [雑誌論文] Effect of heterozygous deletion of WNK1 on the WNK-OSR1/ SPAK-NCC/NKCC1/NKCC2 signal cascade in the kidney and blood vessels (2012)
  • 著者名/発表者名: Susa K, Kita S, Iwamoto T, Yang SS, Lin SH, Ohta A, Sohara E, Rai T, Sasaki S, Alessi DR, Uchida S
  • 雑誌名: Clin. Exp. Nephrol 巻: 16 ページ: 530-538
  • NAID: 10031058026
  • 査読あり
• [雑誌論文] WNK-OSR1/SPAK-NCC signal cascade has circadian rhythm dependent on aldosterone (2012)
  • 著者名/発表者名: Susa K, Sohara E, Isobe K, Chiga M, Rai T, Sasaki S, Uchida S
  • 雑誌名: Biochem. Biophys. Res. Commun 巻: 427 ページ: 743-747
  • 査読あり
• [雑誌論文] Does a β 2-adrenergic receptor-WNK4-Na-Cl co-transporter signal cascade exist in the in vivo kidney? (2012)
  • 著者名/発表者名: Uchida S, Chiga M, Sohara E, Rai T, Sasaki S
  • 雑誌名: Nat. Med 巻: 18 ページ: 1324-1325
  • 査読あり
• [雑誌論文] Close Association of Aquaporin-2 Internalization with Caveolin-1 (2012)
  • 著者名/発表者名: Aoki T., 他
  • 雑誌名: Ａｃｔａ Ｈｉｓｔｏｃｈｅｍｉｃａ ｅｔ Ｃｙｔｏｃｈｅｍｉｃａ 巻: 45 号: 2 ページ: 139-146
  • DOI: 10.1267/ahc.12003
  • NAID: 130001854241
  • ISSN: 0044-5991, 1347-5800
  • 査読あり
• [雑誌論文] Involvement of aquaporin-7 in the cutaneous primary immune response through modulation of antigen uptake and migration in dendritic cells. (2012)
  • 著者名/発表者名: Hara-Chikuma M
  • 雑誌名: FASEB J. 巻: 26 号: 1 ページ: 211-218
  • DOI: 10.1096/fj.11-186627
  • 査読あり
• [雑誌論文] Phosphorylation of Na-Cl cotransporter by OSR1 and SPAK kinases regulates its ubiquitination. (2012)
  • 著者名/発表者名: Hossain Khan MZ
  • 雑誌名: Biochem. Biophys. Res. Commun. 巻: 425 号: 2 ページ: 456-461
  • DOI: 10.1016/j.bbrc.2012.07.124
  • 査読あり
• [雑誌論文] Effects of sevelamer hydrochloride on mortality, lipid abnormality and arterial stiffness in hemodialyzed patients: a propensity-matched observational study. (2012)
  • 著者名/発表者名: Iimori S
  • 雑誌名: Clin. Exp. Nephrol. 巻: 16 号: 6 ページ: 930-937
  • DOI: 10.1007/s10157-012-0640-4
  • 査読あり
• [雑誌論文] Diagnostic usefulness of bone mineral density and biochemical markers of bone turnover in predicting fracture in CKD stage 5D patients - a single-center cohort study. (2012)
  • 著者名/発表者名: Iimori S
  • 雑誌名: Nephrol. Dial. Transplant. 巻: 27 号: 1 ページ: 345-351
  • DOI: 10.1093/ndt/gfr317
  • 査読あり
• [雑誌論文] Applicability of fibroblast growth factor 23 for evaluation of risk of vertebral fracture and chronic kidney disease-mineral bone disease in elderly chronic kidney disease patients. (2012)
  • 著者名/発表者名: Kanda E
  • 雑誌名: BMC Nephrol. 巻: 13 号: 1 ページ: 122-122
  • DOI: 10.1186/1471-2369-13-122
  • 査読あり
• [雑誌論文] Murine colon proteome and characterization of the protein pathways. (2012)
  • 著者名/発表者名: Magdeldin S
  • 雑誌名: BioData Min. 巻: 5 号: 1 ページ: 11-11
  • DOI: 10.1186/1756-0381-5-11
  • 査読あり
• [雑誌論文] Anti-centromere antibody-positive subjects presenting with hypertensive emergency and renal dysfunction in the absence of skin manifestations: a variant of systemic sclerosis or a novel entity? (2012)
  • 著者名/発表者名: Mandai S
  • 雑誌名: Intern.Med. 巻: 51 ページ: 1567-1572
  • 査読あり
• [雑誌論文] Phosphatidylinositol 3-kinase/Akt signaling pathway activates the WNK-OSR1/SPAK-NCC phosphorylation cascade in hyperinsulinemic db/db mice. (2012)
  • 著者名/発表者名: Nishida H
  • 雑誌名: Hypertension 巻: 60 号: 4 ページ: 981-990
  • DOI: 10.1161/hypertensionaha.112.201509
  • 査読あり
• [雑誌論文] Accumulation of autofluorescent storage material in brain is accelerated by ischemia in chloride channel 3 gene-deficient mice. (2012)
  • 著者名/発表者名: Ohtaki H
  • 雑誌名: J. Neurosci. Res. 巻: 90 号: 11 ページ: 2163-2172
  • DOI: 10.1002/jnr.23110
  • 査読あり
• [雑誌論文] A minor role of WNK3 in regulating phosphorylation of renal NKCC2 and NCC co-transporters in vivo (2012)
  • 著者名/発表者名: Oi K
  • 雑誌名: Biology Open 巻: Vol.1 号: 2 ページ: 120-127
  • DOI: 10.1242/bio.2011048
  • 査読あり
• [雑誌論文] Aquaporin 2: from its discovery to molecular structure and medical implications. (2012)
  • 著者名/発表者名: Sasaki S.
  • 雑誌名: Mol. Aspects. Med. 巻: 33 号: 5-6 ページ: 535-546
  • DOI: 10.1016/j.mam.2012.03.004
  • 査読あり
• [雑誌論文] Effect of heterozygous deletion of WNK1 on the WNK-OSR1/ SPAK-NCC/NKCC1/NKCC2 signal cascade in the kidney and blood vessels. (2012)
  • 著者名/発表者名: Susa K,
  • 雑誌名: Clin. Exp. Nephrol. 巻: 16 ページ: 530-538
  • 査読あり
• [雑誌論文] WNK-OSR1/SPAK-NCC signal cascade has circadian rhythm dependent on aldosterone. (2012)
  • 著者名/発表者名: Susa K
  • 雑誌名: Biochem. Biophys. Res. Commun. 巻: 427 号: 4 ページ: 743-747
  • DOI: 10.1016/j.bbrc.2012.09.130
  • 査読あり
• [雑誌論文] Does a β2-adrenergic receptor-WNK4-Na-Cl co-transporter signal cascade exist in the in vivo kidney? (2012)
  • 著者名/発表者名: Uchida S
  • 雑誌名: Nat. Med. 巻: 18 号: 9 ページ: 1324-1325
  • DOI: 10.1038/nm.2809
  • 査読あり
• [雑誌論文] Phenotypes of pseudohypoaldosteronism type II caused by the WNK4 D561A missense mutation are dependent on the WNK-OSR1/SPAK kinase cascade (2011)
  • 著者名/発表者名: Chiga M, Rafiqi FH, Alessi DR, Sohara E, Ohta A, Rai T, Sasaki S, Uchida S
  • 雑誌名: J. Cell Sci 巻: 124 ページ: 1391-1395
  • 査読あり
• [雑誌論文] Regulation of WNK1 kinase by extracellular potassium (2011)
  • 著者名/発表者名: Naito S, Ohta A, Sohara E, Ohta E, Rai T, Sasaki S, Uchida S
  • 雑誌名: Clin. Exp. Nephrol 巻: 15 ページ: 195-202
  • NAID: 10029005115
  • 査読あり
• [雑誌論文] Generation and analyses of R8L barttin knockin mouse (2011)
  • 著者名/発表者名: Nomura N, Tajima M, Sugawara N, Morimoto T, Kondo Y, Ohno M, Uchida K, Mutig K, Bachmann S, Soleimani M, Ohta E, Ohta A, Sohara E, Okado T, Rai T, Jentsch TJ, Sasaki S, Uchida S
  • 雑誌名: Am. J. Physiol. Renal. Physiol 巻: 301
  • 査読あり
• [雑誌論文] Immunolocalization of WNK4 in mouse kidney (2011)
  • 著者名/発表者名: Ohno M, Uchida K, Ohashi T, Nitta K, Ohta A, Chiga M, Sasaki S, Uchida S
  • 雑誌名: Histochem. Cell Biol 巻: 136 ページ: 25-35
  • 査読あり
• [雑誌論文] Acute insulin stimulation induces phosphorylation of the Na-Cl cotransporter in cultured distal mpkDCT cells and mouse kidney (2011)
  • 著者名/発表者名: Sohara E, Rai T, Yang SS, Ohta A, Naito S, Chiga M, Nomura N, Lin SH, Vandewalle A, Ohta E, Sasaki S, Uchida S
  • 雑誌名: PLoS One 巻: 6
  • 査読あり
• [雑誌論文] Severe hyperparathyroidism in a pre-dialysis chronic kidney disease patient treated with a very low protein diet (2011)
  • 著者名/発表者名: Ohta E
  • 雑誌名: J. Bone Miner. Metab. 巻: (published online) 号: 2 ページ: 238-242
  • DOI: 10.1007/s00774-011-0320-6
  • 査読あり
• [雑誌論文] Daily variance of urinary excretion of AQP2 determined by sandwich ELISA method (2011)
  • 著者名/発表者名: Sasaki S
  • 雑誌名: Clin.Exp.Nephrol. 巻: (published online) 号: 3 ページ: 406-410
  • DOI: 10.1007/s10157-011-0574-2
  • NAID: 10030808445
  • 査読あり
• [雑誌論文] Phenotypes of pseudohypoaldosteronism type II caused by the WNK4 D561A missense mutation are dependent on the WNK-OSR1/SPAK kinase cascade (2011)
  • 著者名/発表者名: Chiga M
  • 雑誌名: J. Cell Sci. 巻: Vol.124 号: 9 ページ: 1391-5
  • DOI: 10.1242/jcs.084111
  • 査読あり
• [雑誌論文] The NPC motif of aquaporin-11, unlike the NPA motif of known aquaporins, is essential for full expression of molecular function (2011)
  • 著者名/発表者名: Ikeda M, Andoo A, Shimono M, Takamatsu N, Taki A, Muta K, Matsushita W, Uechi T, Matsuzaki T, Kenmochi N, Takata K, Sasaki S, Ito K, Ishibashi K.
  • 雑誌名: J Biol Chem 巻: 286 号: 5 ページ: 3342-3350
  • DOI: 10.1074/jbc.m110.180968
  • 査読あり
• [雑誌論文] Impaired phosphorylation of Na(+)-K(+)-2Cl(-) cotransporter by oxidative stress-responsive kinase-1 deficiency manifests hypotension and Bartter-like syndrome (2011)
  • 著者名/発表者名: Lin SH
  • 雑誌名: Proc. Natl. Acad. Sci. USA. 巻: Vol.108 号: 42 ページ: 17538-43
  • DOI: 10.1073/pnas.1107452108
  • 査読あり
• [雑誌論文] Regulation of WNK1 kinase by extracellular potassium (2011)
  • 著者名/発表者名: Naito S
  • 雑誌名: Clin. Exp. Nephrol. 巻: Vol.15 号: 2 ページ: 195-202
  • DOI: 10.1007/s10157-010-0378-9
  • 査読あり
• [雑誌論文] Generation and analyses of R8L barttin knockin mouse (2011)
  • 著者名/発表者名: Nomura N
  • 雑誌名: Am. J. Physiol. Renal. Physiol. 巻: Vol.301 号: 2 ページ: F297-F307
  • DOI: 10.1152/ajprenal.00604.2010
  • 査読あり
• [雑誌論文] Dexamethasone increases the phosphorylation of nephrin in cultured podocytes (2011)
  • 著者名/発表者名: Ohashi T
  • 雑誌名: Clin. Exp. Nephrol. 巻: Vol.15 号: 5 ページ: 688-93
  • DOI: 10.1007/s10157-011-0479-0
  • 査読あり
• [雑誌論文] Immunolocalization of WNK4 in mouse kidney (2011)
  • 著者名/発表者名: Ohno M
  • 雑誌名: Histochem. Cell Biol. 巻: Vol.136 号: 1 ページ: 25-35
  • DOI: 10.1007/s00418-011-0827-x
  • 査読あり
• [雑誌論文] Acute insulin stimulation induces phosphorylation of the Na-Cl cotransporter in cultured distal mpkDCT cells and mouse kidney (2011)
  • 著者名/発表者名: Sohara E
  • 雑誌名: PLoS One. 巻: Vol.6
  • 査読あり
• [雑誌論文] Phosphorylation of aquaporin-2 regulates its water permeability (2010)
  • 著者名/発表者名: Eto K, Noda Y, Horikawa S, Uchida S, Sasaki S
  • 雑誌名: J. Biol. Chem 巻: 285 ページ: 40777-40784
  • 査読あり
• [雑誌論文] Effect of angiotensin II on the WNK-OSR1/SPAK-NCC phosphorylation cascade in cultured mpkDCT cells and in vivo mouse kidney (2010)
  • 著者名/発表者名: Talati G, Ohta A, Rai T, Sohara E, Naito S, Vandewalle A, Sasaki S, Uchida S
  • 雑誌名: Biochem. Biophys. Res. Commun 巻: 393 ページ: 844-848
  • 査読あり
• [雑誌論文] Mechanisms for hypercalciuria in pseudohypoaldosteronism type II-causing WNK4 knock-in mice (2010)
  • 著者名/発表者名: Yang SS, Hsu YJ, Chiga M, Rai T, Sasaki S, Uchida S, Lin SH
  • 雑誌名: Endocrinology 巻: 151 ページ: 1829-1836
  • 査読あり
• [雑誌論文] Phosphorylation of aquaporin-2 regulates its water permeability. (2010)
  • 著者名/発表者名: Eto K
  • 雑誌名: J.Biol.Chem. 巻: Vol.285 ページ: 40777-40784
  • 査読あり
• [雑誌論文] Assessment and significance of abdominal aortic calcification in chronic kidney disease. (2010)
  • 著者名/発表者名: Hanada S
  • 雑誌名: Nephrol.Dial.Transplant. 巻: Vol.25 ページ: 1889-1895
  • 査読あり
• [雑誌論文] Aquaporins in kidney pathophysiology. (2010)
  • 著者名/発表者名: Noda Y
  • 雑誌名: Nat.Rev.Nephrol. 巻: Vol.6 ページ: 168-178
  • 査読あり
• [雑誌論文] Calcium and calcimimetics regulate paracellular Na+ transport in the thinascending limb of Henle's loop in mouse kidney. (2010)
  • 著者名/発表者名: Sugawara N
  • 雑誌名: Pflugers Arch. 巻: Vol.460 ページ: 197-205
  • 査読あり
• [雑誌論文] Effect of angiotensin I1 on the WNK-OSR1/SPAK-NCC phosphorylation cascadein cultured mpkDCT cells and in vivo mouse kidney. (2010)
  • 著者名/発表者名: Talati G
  • 雑誌名: Biochem.Biophys.Res.Commun. 巻: Vol.393 ページ: 844-848
  • 査読あり
• [雑誌論文] Generation and analysis of the thiazide-sensitive Na+ -Cl- cotransporter(Ncc/Slcl2a3) Ser707X knockin mouse as a model of Gitelman syndrome. (2010)
  • 著者名/発表者名: Yang SS
  • 雑誌名: Hum.Mutat. 巻: Vol.31 ページ: 1304-1315
  • 査読あり
• [雑誌論文] SPAK-knockout mice manifest Gitelman syndrome and impaired vasoconstriction. (2010)
  • 著者名/発表者名: Yang SS
  • 雑誌名: J.Am.Soc.Nephrol. 巻: Vol.21 ページ: 1868-1877
  • 査読あり
• [雑誌論文] Mechanisms for hypercalciuria in pseudohypoaldosteronism type II-causing WNK4knock-in mice. (2010)
  • 著者名/発表者名: Yang SS
  • 雑誌名: Endocrinology 巻: Vol.151 ページ: 1829-1836
  • 査読あり
• [学会発表] Analyses of KLHL3 mutants that cause Pseudohypoaldosteronism type II (2013)
  • 著者名/発表者名: Mori Y, Wakabayashi M, Mori T, Araki Y, Sohara E, Rai T, Sasaki S, Uchida S
  • 学会等名: The 46^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Atlanta
  • 年月日: 2013-11-09
• [学会発表] KLHL2 interacts with and ubiquitinates WNK kinases (2013)
  • 著者名/発表者名: Takahashi D, Mori T, Wakabayashi M, Mori Y, Susa K, Zeniya M, Sohara E, Rai T, Sasaki S, Uchida S
  • 学会等名: The 46^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Atlanta
  • 年月日: 2013-11-09
• [学会発表] Impaired KLHL3-mediated ubiquitination of WNK4 activates OSR1 and SPAK kinases-NaCl cotransporter (NCC) signaling and causes hypertension (2013)
  • 著者名/発表者名: Wakabayashi M, Mori T, Isobe K, Sohara E, Susa K, Araki Y, Chiga M, Kikuchi E, Nomura N, Mori Y, Rai T, Sasaki S, Uchida S
  • 学会等名: The 46^<st> Annual Meeting of American Society of Nephrology
  • 発表場所: Atlanta
  • 年月日: 2013-11-09
• [学会発表] WNK3 Regulates Blood Pressure Through the Regulation of Vascular OSR1/SPAK-NKCC1 Phosphorylation Cascade (2013)
  • 著者名/発表者名: Zeniya M, Sohara E, Kita S, Iwamoto T, Susa K, Mori T, Oi K, Chiga M, Takahashi D, Yang SS, Lin SH, Rai T, Sasaki S, Uchida S
  • 学会等名: The 46^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Atlanta
  • 年月日: 2013-11-09
• [学会発表] Aberrant Glycosylation and Localization of Polycystin-1 Cause Polycystic Kidney in AQP11-Knockout Mice (2013)
  • 著者名/発表者名: Inoue Y, Sohara E, Kobayashi K, Rai T, Ishibashi K, Horie S, Su X, Zhou J, Sasaki S, Uchida S
  • 学会等名: The 46^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Atlanta
  • 年月日: 2013-11-07
• [学会発表] Clinical Significance of Urinary Thiazide-Sensitive Na-Cl Cotransporter (NCC) Measurement by Newly Development Enzyme-Linked Immunosorbent Assays (2013)
  • 著者名/発表者名: Isobe K, Mori T, Sohara E, Rai T, Sasaki S, Uchida S
  • 学会等名: The 46^<th> Annual meeting of American Society of Nephrology
  • 発表場所: Atlanta
  • 年月日: 2013-11-07
• [学会発表] Chemical library screening for WNK signaling inhibitors by using fluorescent correlation spectroscopy (2013)
  • 著者名/発表者名: Mori T, Eriko K, Sohara E, Rai T, Sasaki S, Uchida S
  • 学会等名: The 46^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Atlanta
  • 年月日: 2013-11-07
• [学会発表] Low salt intake decreased transcription and protein level of KLHL3 in mouse kidney (2013)
  • 著者名/発表者名: Susa K, Sohara E, Zeniya M, Rai T, Sasaki S, Uchida S
  • 学会等名: The 46^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Atlanta
  • 年月日: 2013-11-07
• [学会発表] Ser-261 phosphorylation is an endocytic signal of aquaporin-2 water channel (2013)
  • 著者名/発表者名: Yui N, Uchida S, Sasaki S
  • 学会等名: The 46^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Atlanta, USA
  • 年月日: 2013-11-07
• [学会発表] Chemical library screening for WNK signaling inhibitors by using fluorescent correlation spectroscopy. (2013)
  • 著者名/発表者名: Mori T, Eriko K, Sohara E, Rai T, Sasaki S, Uchida S.
  • 学会等名: The 46th Annual meeting of American Society of Nephrology
  • 発表場所: アトランタ, アメリカ合衆国
• [学会発表] Aberrant Glycosylation and Localization of Polycystin-1 Cause Polycystic Kidney in AQP11-Knockout Mice. (2013)
  • 著者名/発表者名: Inoue Y, Sohara E, Kobayashi K, Rai T, Ishibashi K, Horie S, Su X, Zhou J, Sasaki S, Uchida S.
  • 学会等名: The 46th Annual meeting of American Society of Nephrology
  • 発表場所: アトランタ, アメリカ合衆国
• [学会発表] Ser-261 phosphorylation is an endocytic signal of aquaporin-2 water channel. (2013)
  • 著者名/発表者名: Yui N, Uchida S, Sasaki S.
  • 学会等名: The 46th Annual meeting of American Society of Nephrology
  • 発表場所: アトランタ, アメリカ合衆国
• [学会発表] Clinical Significance of Urinary Thiazide-Sensitive Na-Cl Cotransporter (NCC) Measurement by Newly Development Enzyme-Linked Immunosorbent Assays. (2013)
  • 著者名/発表者名: Isobe K, Mori T, Sohara E, Rai T, Sasaki S, Uchida S.
  • 学会等名: The 46th Annual meeting of American Society of Nephrology
  • 発表場所: アトランタ, アメリカ合衆国
• [学会発表] Impaired KLHL3-mediated ubiquitination of WNK4 activates OSR1 and SPAK kinases-NaCl cotransporter (NCC) signaling and causes hypertension. (2013)
  • 著者名/発表者名: Wakabayashi M, Mori T, Isobe K, Sohara E, Susa K, Araki Y, Chiga M, Kikuchi E, Nomura N, Mori Y, Rai T, Sasaki S, Uchida S.
  • 学会等名: The 46th Annual meeting of American Society of Nephrology
  • 発表場所: アトランタ, アメリカ合衆国
• [学会発表] Hereditary nephrogenic diabetes insipidus (NDI) in Japanese patients : Analysis of 73 families (2012)
  • 著者名/発表者名: Sasaki S, Chiga M, Kikuchi E, Uchida S
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
  • 年月日: 2012-11-02
• [学会発表] WNK4-OSR1/SPAK-NCC signal cascade has circadian rhythm dependent on aldosterone (2012)
  • 著者名/発表者名: Susa K, Sohara E, Isobe K, Chiga M, Rai T, Sasaki S, Uchida S
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
  • 年月日: 2012-11-02
• [学会発表] Chemical library screening for direct SPAK inhibitors by a newly developed ELISA system (2012)
  • 著者名/発表者名: Kikuchi E, Mori T, Isobe K, Sohara E, Rai T, Sasaki S, Uchida S
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
  • 年月日: 2012-11-01
• [学会発表] Generation and analysis of WT-WNK4 transgenic mice revealed the physiological role of WNK4 (2012)
  • 著者名/発表者名: Mori T, Sohara E, Rai T, Sasaki S, Uchida S
  • 学会等名: The 45th Annual Meeting of American Society of Nephrology
  • 発表場所: San Diego (USA)
  • 年月日: 2012-11-01
• [学会発表] The size and viability of mesothelial cells affect peritoneal membrane function. (2012)
  • 著者名/発表者名: Akazawa M
  • 学会等名: The 45th Annual meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Phosphorylation of Na-Cl cotransporter by OSR1 and SPAK kinases regulates its ubiquitination. (2012)
  • 著者名/発表者名: Hossain Khan MZ
  • 学会等名: The 45th Annual meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Impaired trafficking of polycystin-1 may be a key mechanism of cyst formation in the aquaporin-11 knockout mouse. (2012)
  • 著者名/発表者名: Inoue Y
  • 学会等名: The 45th Annual meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Development of sandwich enzyme linked immunosorbent assay (ELISA) for measurement of urinary total and phosphorylated Na-Clcotransporter (NCC) protein. (2012)
  • 著者名/発表者名: Isobe K
  • 学会等名: The 45th Annual meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Rate of change in fibroblast growth factor 23 level predicts the progression of chronic kidney disease in elderly patients. (2012)
  • 著者名/発表者名: Kanda E
  • 学会等名: The 45th Annual meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] A new high-performance liquid chromatography showed that icodextrin dialysate favorably improved lipid profiles in peritoneal dialysis patients. (2012)
  • 著者名/発表者名: Kanda E
  • 学会等名: The 45th Annual meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Chemical library screening for direct SPAK inhibitors by a newly developed ELISA system. (2012)
  • 著者名/発表者名: Kikuchi E
  • 学会等名: The 45th Annual meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Generation and analysis of WT-WNK4 transgenic mice revealed the physiological role of WNK4. (2012)
  • 著者名/発表者名: Mori T
  • 学会等名: The 45th Annual meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Hereditary nephrogenic diabetes insipidus (NDI) in Japanese patients: Analysis of 73 families. (2012)
  • 著者名/発表者名: Sasaki S
  • 学会等名: The 45th Annual meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] WNK4-OSR1/SPAK-NCC signal cascade has circadian rhythm dependent on aldosterone. (2012)
  • 著者名/発表者名: Susa K
  • 学会等名: The 45th Annual meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Dietary salt intake and angiotensin II regulates WNK-SPAK-NKCC1 phosphorylation cascade in mouse aorta. (2012)
  • 著者名/発表者名: Zeniya M
  • 学会等名: The 45th Annual meeting of American Society of Nephrology
  • 発表場所: San Diego Convention Center
• [学会発表] Generation and analyses of AQP11 BAC transgenic mice (2011)
  • 著者名/発表者名: Inoue Y, Sohara E, Kobayashi K, Rai T, Ishibashi K, Sasaki S, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
  • 年月日: 2011-11-12
• [学会発表] High throughput screening of drugs that inhibit WNK-OSR1/SPAK signaling cascade (2011)
  • 著者名/発表者名: Mori T, Ohta A, Sohara E, Rai T, Sasaki S, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
  • 年月日: 2011-11-12
• [学会発表] Renal phenotype of WNK3 knockout mouse (2011)
  • 著者名/発表者名: Oi K, Sohara E, Rai T, Chiga M, Alessi DR, Sasaki S, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
  • 年月日: 2011-11-12
• [学会発表] Chemical library screening for drugs to correct intracellular mislocalization of R8L mutant barttin (2011)
  • 著者名/発表者名: Nomura N, Naito S, Sohara E, Rai T, Sasaki S, Uchida S
  • 学会等名: The 44th Annual Meeting of American Society of Nephrology
  • 発表場所: Philadelphia (USA)
  • 年月日: 2011-11-11
• [学会発表] Generation and analyses of AQP11 BAC transgenic mice (2011)
  • 著者名/発表者名: Inoue Y
  • 学会等名: The 44^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Pennsylvania Convention Center, Philadelphia, U.S.A.
• [学会発表] Development of new systems to measure total and phosphorylated Na-Cl cotransporter (NCC) protein in human urine (2011)
  • 著者名/発表者名: Isobe K
  • 学会等名: The 44^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Pennsylvania Convention Center, Philadelphia, U.S.A.
• [学会発表] Urinary excretion of Na-ClCotransporter in exosomes is increased by high salt diet as well as low salt diet (2011)
  • 著者名/発表者名: Kahn MZH
  • 学会等名: The 44^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Pennsylvania Convention Center, Philadelphia, U.S.A.
• [学会発表] High throughput screening of drugs that inhibit WNK-OSR1/SPAK signaling cascade (2011)
  • 著者名/発表者名: Mori T
  • 学会等名: The 44^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Pennsylvania Convention Center, Philadelphia, U.S.A.
• [学会発表] Increased Na-Cl cotransporter phosphorylation in hyperinsulinemic db/db mice is regulated by insulin/PI3K pathway (2011)
  • 著者名/発表者名: Nishida H
  • 学会等名: The 44^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Pennsylvania Convention Center, Philadelphia, U.S.A.
• [学会発表] Chemical library screening for drugs to correct intracellular mislocalization of R8L mutant barttin (2011)
  • 著者名/発表者名: Nomura N
  • 学会等名: The 44^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Pennsylvania Convention Center, Philadelphia, U.S.A.
• [学会発表] Renal phenotype of WNK3 knockout mouse (2011)
  • 著者名/発表者名: Oi K
  • 学会等名: The 44^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Pennsylvania Convention Center, Philadelphia, U.S.A.
• [学会発表] Acute insulin stimulation induces phosphorylation of the Na-Cl cotransporter in cultured distal mpkDCT cells and mouse kidney (2011)
  • 著者名/発表者名: Sohara E
  • 学会等名: The 44^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Pennsylvania Convention Center, Philadelphia, U.S.A.
• [学会発表] WNK-OSR1/SPAK-SLC12A phosphorylation cascade in the WNK1 (+/-) mice (2011)
  • 著者名/発表者名: Susa K
  • 学会等名: The 44^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Pennsylvania Convention Center, Philadelphia, U.S.A.
• [学会発表] Increased protein abundance of the mutant WNK4 may be a cause of the increased WNK4 kinase activity in the mouse model of pseudohypoaldosteronism type II (2011)
  • 著者名/発表者名: Wakabayashi M
  • 学会等名: The 44^<th> Annual Meeting of American Society of Nephrology
  • 発表場所: Pennsylvania Convention Center, Philadelphia, U.S.A.
• [学会発表] Treatment of Bartter syndrome type IV caused by R8L barttin mutation (2010)
  • 著者名/発表者名: Nomura N, Masato T, Ohta E, Ohta A, Sohara E, Rai T, Sasaki S, Uchida S
  • 学会等名: The 43nd Annual Meeting of American Society of Nephrology
  • 発表場所: Denver (USA)
  • 年月日: 2010-11-10
• [学会発表] 腎性尿崩症 (2010)
  • 著者名/発表者名: 佐々木成
  • 学会等名: 第53回日本腎臓学会学術総会ワークショップ
  • 発表場所: 神戸国際会議場, 神戸
  • 年月日: 2010-06-16
• [学会発表] Evidence based CKD guideline in Japan. (2010)
  • 著者名/発表者名: Sasaki S
  • 学会等名: The 12^<th> Asian Pacific Congress of Nephrology
  • 発表場所: COEX, Seoul, Korea
  • 年月日: 2010-06-05
• [学会発表] Diagnostic usefulness of bone mineral density in predicting vertebral fracture and abdominal aortic calcification in CKD stage 5D patients. (2010)
  • 著者名/発表者名: Iimori S
  • 学会等名: The 43^<nd> Annual Meeting of American Society of Nephrology
  • 発表場所: Colorado Convention Center, Denver, U.S.A.
• [学会発表] Disruption of mouse AQP11 induces endoplasmic reticulum stress, followed by a poptosis and cellular proliferation, in the proximal tubule cells of the kidney. (2010)
  • 著者名/発表者名: Kobayashi K
  • 学会等名: The 43^<nd> Annual Meeting of American Society of Nephrology
  • 発表場所: Colorado Convention Center, Denver, U.S.A.
• [学会発表] Treatment of Bartter syndrome type IV caused by R8L barttin mutation. (2010)
  • 著者名/発表者名: Nomura N
  • 学会等名: The 43^<nd> Annual Meeting of American Society of Nephrology
  • 発表場所: Colorado Convention Center, Denver, U.S.A.
• [学会発表] The effect of sevelamer hydrochloride on arterial stiffness and left ventricular fun xton.A prospective cohort study. (2010)
  • 著者名/発表者名: Tsukamoto Y
  • 学会等名: The 43^<nd> Annual Meeting of American Society of Nephrology
  • 発表場所: Colorado Convention Center, Denver, U.S.A.
• [図書] Chapter 4. Regulation of Water Balance: Urine Concentration and Dilution. Diseases of the Kidney & Urinary Tract, Ninth Edition. (2012)
  • 著者名/発表者名: Noda Y, Sasaki S.
  • 出版者: Lippincott Williams & Wilkins
• [図書] バゾプレシンのV2作用:AQP2調節と水代謝.バゾプレシンと受容体拮抗-その基礎と臨床- (2011)
  • 著者名/発表者名: 佐々木成
  • 出版者: メディカルビュー社
• [図書] 腎臓内科学 (2010)
  • 著者名/発表者名: 佐々木成編集
  • 総ページ数: 209
  • 出版者: シュプリンガー・ジャパン
• [図書] 体液とその組成ポケット輸液マニュアル(改訂版) (2010)
  • 著者名/発表者名: 佐々木成(北岡建樹編集)
  • 出版者: 羊土社
• [備考] 東京医科歯科大学腎臓内科学ホームページ
  • URL: http://www.tmd.ac.jp/grad/kid/kid-J.htm
• [備考] 東京医科歯科大学腎臓内科学
  • URL: http://www.tmd.ac.jp/grad/kid/kid-J.htm
• [備考] 東京医科歯科大学腎臓内科学ホームページ
  • URL: http://www.tmd.ac.jp/grad/kid/kid-J.htm
• [備考]
  • URL: http://www.tmd.ac.jp/grad/kid/kid-J.htm
• [備考] 東京医科歯科大学腎臓内科ホームページ
  • URL: http://www.tmd.ac.jp/grad/kid/kid-J.htm
• [産業財産権] タンパク質を含む生体試料の前処理方法 (2011)
  • 発明者名: 佐々木成、大本安一、森豊樹、岩田房子、村口正宏
  • 権利者名: 大塚製薬株式会社、国立大学法人東京医科歯科大学
  • 産業財産権番号: 2011-176272
  • 出願年月日: 2011-08-11