| 研究課題/領域番号 |
22F20708
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| 研究種目 |
特別研究員奨励費
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| 配分区分 | 補助金 |
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| 応募区分 | 外国 |
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| 審査区分 |
小区分28050:ナノマイクロシステム関連
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| 研究機関 | 東京大学 |
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研究代表者 |
竹内 昌治 東京大学, 生産技術研究所, 教授 (90343110)
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| 研究分担者 |
LISI FABIO 東京大学, 生産技術研究所, 外国人特別研究員
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| 研究期間 (年度) |
2022-04-22 – 2023-03-31
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| 研究課題ステータス |
完了 (2022年度)
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| 配分額 *注記 |
1,200千円 (直接経費: 1,200千円)
2022年度: 1,200千円 (直接経費: 1,200千円)
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| キーワード | Gold nanoparticles / hydrogel / controlled release / DNA / photothermal effect / microfluidic / cell culture / epithelial cells |
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| 研究開始時の研究の概要 |
This research focuses of controlling the functional and biochemical properties of commercial hydrogels by using nanoparticles. These nanomaterials can be used for the localised delivery of signalling molecules that can influence cells embedded in the hydrogel, or as sensing elements to detect biomarkers of specific diseases. Delivery is triggered by using red or near-infrared light, a method that does not damage the cells and could potentially be used in-vivo. The results will correlate the dynamic changes in the hydrogel microenvironment to local composition, cell morphology and behaviour.
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| 研究実績の概要 |
The focus of my research was the development of nanoparticle systems that could be introduced into commercial hydrogels to tune their properties. One type of nanoparticle that I worked with are fluorescent quantum dots. To use these particles in hydrogel systems, it is essential to optimize their stability and optical properties in physiologically relevant conditions. I conducted a fundamental study, showing how previously published articles did not rigorously investigate the surface chemistry of these particles. The results of this investigation were published in the journal ACS Applied Nano Materials and will be presented to a conference in June 2023.
Another nanoparticle system that I continued developing was based on gold nanorods and DNA. Gold nanorods can very efficiently generate heat when irradiated by a red laser, and the resulting heat is transferred to the surrounding environment. When double strands of DNA are immobilized on the rods’ surface, the heat causes DNA melting and subsequent release of one strand into the surrounding media. This mechanism was confirmed using fluorophore-labelled DNA, and the plan was to use a laser for the targeted release of a growth factor in 3D cultures of endothelial cells. Endothelial cells spheroids were produced and used in a sprouting assay to test the release of growth factor from the Gold-DNA particles. More tests will be required to fully characterize the response of this system to laser irradiation and the resulting morphological changes on the endothelial cells.
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| 現在までの達成度 (段落) |
令和4年度が最終年度であるため、記入しない。
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| 今後の研究の推進方策 |
令和4年度が最終年度であるため、記入しない。
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