| 研究開始時の研究の概要 |
Bioprinting has emerged in the field of tissue engineering to meet the need for precise organization of biological components and overcome limitations of conventional techniques. Previous work demonstrated the benefits of in situ laser-assisted bioprinting (LAB) on vascularization and bone regeneration, but optimizations of the process have to be considered, especially regarding mechanical and biological properties of the hydrogel used. This collaborative project aims to develop a tailored hydrogel with optimized mechanical and biological properties in order to be used as bioink and biopaper.
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| 研究実績の概要 |
Gelatin (Gel5wt%)-sheets incorporating bioactive glasses (BG0wt%, 1wt%, 3wt% or 5wt%) were fabricated and underwent a dehydrothermal crosslinking (CL120°C, CL140°C or CL160°C 24h). The condition “Gel5wt% BG1wt% CL160°C” allowed to obtain the best results in terms of physical (porosity, pore size, swelling ratio), mechanical (Young modulus) and biological (viability, proliferation, ALP activity, calcium deposition) properties, in monoculture and coculture models. After immersion into simulated body fluid, Ca/P molar ratios measured using EDX analysis were between 1.72 and 1.77, suggesting a good bioactivity of the biomaterial. In coculture, this formulation supported the formation of microvascular networks. Finally, this novel biopaper was successfully used as a receiving substrate for Laser-Assisted Bioprinting technology, but also adapted to 3D-moulding and stacking approaches to generate 3D constructs.
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