| 研究課題/領域番号 |
22H00360
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| 研究種目 |
基盤研究(A)
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| 配分区分 | 補助金 |
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| 応募区分 | 一般 |
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| 審査区分 |
中区分38:農芸化学およびその関連分野
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| 研究機関 | 名古屋大学 |
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研究代表者 |
Frommer Wolf 名古屋大学, トランスフォーマティブ生命分子研究所, 客員教授 (70795575)
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| 研究期間 (年度) |
2022-04-01 – 2025-03-31
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| 研究課題ステータス |
交付 (2024年度)
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| 配分額 *注記 |
42,510千円 (直接経費: 32,700千円、間接経費: 9,810千円)
2024年度: 14,040千円 (直接経費: 10,800千円、間接経費: 3,240千円)
2023年度: 13,390千円 (直接経費: 10,300千円、間接経費: 3,090千円)
2022年度: 15,080千円 (直接経費: 11,600千円、間接経費: 3,480千円)
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| キーワード | Phytohormone / Gibberelllic acid / Sugar / SWEETs / Transporter / Gibberellic acid |
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| 研究開始時の研究の概要 |
GA and sugars can have synergistic effects on plant growth and yield, seed storage mobilization, fertility, and stress tolerance. The dual specificity of SWEETs for sugars and GA is possible nexus to understand GA and sugar interactions. We will here develop approaches and novel tools to dissect the contribution of sugars and GA and lay the basis for engineering improved plants with respect to the trails.
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| 研究実績の概要 |
Several SWEET transporters have been demonstrated to mediate transport of the plant hormone gibberellin (GA). The close physiological relationship between sucrose and GA raised the question of whether a functional interaction exists between these two substrates and whether they are both physiologically relevant. To address these inquiries, molecular docking and molecular dynamics simulations were conducted to predict the amino acids responsible for each substrate binding. Transport activity assays were then performed, which revealed mutants that selectively transport either sucrose or GA. These mutants were used to determine the physiological contribution of two different substrates facilitated by the same transporter. We found the sugar transport activity of SWEET13, not GA are responsible for pollen development.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Through the creation of substrate-specific transporters and their implementation in plant systems, our findings suggest that the sucrose transport functionality of SWEET13, rather than GA, plays a pivotal role in pollen development.
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| 今後の研究の推進方策 |
We will continue to explore the physiological relevance of sugar and GA transport activity of SWEETs. Additionally, we will endeavor to identify novel factors implicated in the governance of SWEET transport activity, with the object of comprehending the molecular mechanism of selectivity and activity regulation. Furthermore, we aim to develop a highly sensitive GA biosensor to facilitate an understanding of SWEET-mediated GA dynamics in planta. The signaling network of GA will also be investigated using a synthetic chemistry approach.
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