研究課題/領域番号 |
22H01343
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研究種目 |
基盤研究(B)
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配分区分 | 補助金 |
応募区分 | 一般 |
審査区分 |
小区分17050:地球生命科学関連
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研究機関 | 東京工業大学 |
研究代表者 |
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研究分担者 |
掛川 武 東北大学, 理学研究科, 教授 (60250669)
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研究期間 (年度) |
2022-04-01 – 2025-03-31
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研究課題ステータス |
交付 (2023年度)
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配分額 *注記 |
17,290千円 (直接経費: 13,300千円、間接経費: 3,990千円)
2023年度: 5,460千円 (直接経費: 4,200千円、間接経費: 1,260千円)
2022年度: 5,980千円 (直接経費: 4,600千円、間接経費: 1,380千円)
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キーワード | Enzyme / Isotope / Origin of life / Energy conservation / Evolution / enzyme / thioester / anaerobe / origin of life / sulfur isotope |
研究開始時の研究の概要 |
Sulfur is required by all life in energy reactions, but there has never been a direct comparative biochemical study about the evolutionary history of sulfur verses phosphate utilization with central and ancient metabolic enzymes. We now seek to expand our analysis evolutionarily to investigate the relative ages of sulfur and phosphate based energy harvesting strategies. In addition to this evolutionary biochemical approach, we will integrate our laboratory data with those of Earth history, to search for clues of ancient energy harvesting on the early Earth.
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研究実績の概要 |
All life requires energy. One major pathway of energy harvesting in biology is substrate level phosphorylation, which is carried out by the enzyme glyceraldehyde 3-phosphate dehydrogenase. How this enzyme evolved, and what its original chemical reactivity was remains unknown.
In this year we successfully surveyed the chemical reactivity of the enzyme glyceraldehyde 3-phosphate dehydrogenase from a thermophilic bacterium. To our surprise, we have identified a new enzymatic reaction which to our knowledge has not been reported before. In addition we find that the conditions of the assay which are being uniquely manipulated within our laboratory can change the reaction kinetics of the enzyme activity in surprising and still not completely understood ways. We are proceeding to draft these results into a manuscript to share with the scientific community.
Our results are far reaching. First, they give new insights into the central energy harvesting enzyme of glycolysis which is widely distributed across the tree of life. Second, our results provide new insights which can be used to discuss the possible ancestral activity of this fascinating enzyme which accomplishes substrate phosphorylation.
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現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
We were able to accomplish the goals of our first year research proposal but also uncovered results more interesting than originally planned.
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今後の研究の推進方策 |
In our future work we will follow the original proposal and attempt to incorporate our results into our understanding of the Earth-Life geochemical record.
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