| 研究課題/領域番号 |
22K05330
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| 研究種目 |
基盤研究(C)
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| 配分区分 | 基金 |
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| 応募区分 | 一般 |
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| 審査区分 |
小区分37020:生物分子化学関連
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| 研究機関 | 香川大学 |
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研究代表者 |
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| 研究期間 (年度) |
2022-04-01 – 2027-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
4,290千円 (直接経費: 3,300千円、間接経費: 990千円)
2026年度: 130千円 (直接経費: 100千円、間接経費: 30千円)
2025年度: 130千円 (直接経費: 100千円、間接経費: 30千円)
2024年度: 130千円 (直接経費: 100千円、間接経費: 30千円)
2023年度: 390千円 (直接経費: 300千円、間接経費: 90千円)
2022年度: 3,510千円 (直接経費: 2,700千円、間接経費: 810千円)
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| キーワード | nanopore |
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| 研究開始時の研究の概要 |
Efficient discovery of new biological nanopores and conversion of existing ones into new ones is of most significance in technology development. The proposed multidisciplinary research program is to explore new strategies to expand the scope of biological nanopores for sensing biomolecules.
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| 研究実績の概要 |
The establishments of the UPLC-MS system and the Benchtop NMR system have enabled the scale-up production of the essential chemicals. Various methods for the UPLC-MS system and the Benchtop NMR system have been generated and optimised. Large-scale expression of the target protein has also been achieved. The protocols for large-scale affinity chromatography, ion-exchange chromatography and size-exclusion chromatography with the AKTAgo FPLC system have been extensively explored and optimised. SDS-PAGE characterisation of the fractions from each chromatography purification gave the desired purity in good quantity. Thus, scale-up protein production and purification have also been achieved. The subsequent scale-up production of nanopores and follow-up purification is in progress. Various methods are being examined for the best results. Initial measurements with the relevant biophysical instruments are encouraging. Hopefully, desired nanopores with very high purity would be obtained soon so that the desired properties could be achieved.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
The delays in FY 2022 have led to slight delays in FY2023.
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| 今後の研究の推進方策 |
The project is expected to progress smoothly from now on.
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