| 研究課題/領域番号 |
22K06430
|
|---|
| 研究種目 |
基盤研究(C)
|
| 配分区分 | 基金 |
|---|
| 応募区分 | 一般 |
|---|
| 審査区分 |
小区分46010:神経科学一般関連
|
|---|
| 研究機関 | 京都大学 |
|---|
研究代表者 |
|
|---|
| 研究分担者 |
土井 大輔 京都大学, iPS細胞研究所, 特定拠点講師 (10587851)
|
|---|
| 研究期間 (年度) |
2022-04-01 – 2025-03-31
|
| 研究課題ステータス |
交付 (2023年度)
|
| 配分額 *注記 |
4,030千円 (直接経費: 3,100千円、間接経費: 930千円)
2024年度: 1,170千円 (直接経費: 900千円、間接経費: 270千円)
2023年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2022年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
|
|---|
| キーワード | Parkinson's Disease / Magnetic Nanoparticle / Cell Replacment / Stem Cells / Dopaminergic Neurons / Dopaminergic neurons / Stem cells (iPSCs) / Magnetic nanoparticles / Halbach magnet / Axon guidance / CNS regeneration / Parkinson's disease / Nanotechnology / Cell Replacement Therapy / Magnetic Nanoparticles / Pluripotent Stem Cells / Neuronal Regeneration |
|---|
| 研究開始時の研究の概要 |
To rewire the brain, we aim for the remote-controlled guidance of neurite growth by magnetic nanoparticles (MNPs). When an external magnetic field is applied, these MNPs generate forces at the cell membrane which promote neurite growth and increase neurite extension in the direction of the magnetic field.
The aim is to adept this system for single induced dopaminergic neurons and neurospheres. We will use the obtained knowledge and transplant the MNP-loaded neurospheres in Parkinson’s disease model rats to rewire the disconnected brain regions.
|
| 研究実績の概要 |
In the FY2023, large efforts were made towards the magnetic manipulation of nanoparticle-loaded dopaminergic neurospheres. In close collaboration with the Raffa Lab (Pisa, Italy) we were able to show the asymmetric neurite growth in direction of the magnetic field.
As the transition to animal models requires further optimization, we performed the magnetic manipulation in ex vivo cultures. These cultures are 350 um thick sections of the mouse brain. Nanoparticle=loaded spheres were grafted into these ex vivo cultures. We were able to see neurite growth in the direction of the magnetic field.
In summary, in the FY2023, we were able to translate our approach to the neurosphere model and were able to show that the stretch growth is still presence in an organotypic environment.
|
| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
As mentioned above, we were able to tackle the two major points proposed for FY2023. On the one hand, the magnetic manipulation of neurospheres was done. On the other hand, the first step towards the application of our approach in vivo was tested. Now, the actual application in vivo can be tested.
|
| 今後の研究の推進方策 |
The first step is generating more data with our neurosphere and ex vivo ex vivo model. After optimizing the parameters, we can translate the approach to the animal model. Here, the biggest task is to finalize designing an appropriate magnetic applicator.
|
