| 研究課題/領域番号 |
22K06980
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| 研究種目 |
基盤研究(C)
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| 配分区分 | 基金 |
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| 応募区分 | 一般 |
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| 審査区分 |
小区分49020:人体病理学関連
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| 研究機関 | 広島大学 |
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研究代表者 |
AMATYA VISHWA・J 広島大学, 医系科学研究科(医), 講師 (90403625)
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| 研究分担者 |
櫛谷 桂 広島大学, 医系科学研究科(医), 助教 (00508179)
武島 幸男 広島大学, 医系科学研究科(医), 教授 (70236462)
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| 研究期間 (年度) |
2022-04-01 – 2025-03-31
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| 研究課題ステータス |
交付 (2022年度)
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| 配分額 *注記 |
4,160千円 (直接経費: 3,200千円、間接経費: 960千円)
2024年度: 910千円 (直接経費: 700千円、間接経費: 210千円)
2023年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円)
2022年度: 1,560千円 (直接経費: 1,200千円、間接経費: 360千円)
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| キーワード | Mesothelioma / Chemoresistant / lncRNA / Microarray / mesothelioma / lncRNA expression / chemoresistant / microarray / cell lines |
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| 研究開始時の研究の概要 |
Cisplatin plus pemetrexed chemotherapy in mesothelioma patients has not improved the survival because of acquisition of chemoresistance.
Recently, lncRNAs are reported to be involved in chemoresistance to cisplatin and pemetrexed in human cancers. However, the mechanism in mesothelioma cell is not yet clear.
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| 研究実績の概要 |
Malignant mesothelioma is the aggressive cancer. Cisplatin plus pemetrexed chemotherapy in mesothelioma patients has not improved the outcome, due to acquisition of chemoresistance by malignant mesothelioma, which is not yet fully known. Recently, long non-coding RNAs are reported to be involved in chemoresistance mechanism. However, the mechanism in mesothelioma cell is not yet clear.
We analyzed gene expression of chemoresistant and chemosensitive mesothelioma using whole gene expression analysis.
After Total RNA expression, the samples were hybridized in Genechip and data obtained from the genechip scanner was analyzed with transcriptome Analysis Consloe
We found 2 fold differential expression of 209 noncoding gene between two groups, with 78 upregulated and 131 downregulated in chemoresistant mesothelioma.
Further analysis is ungoing.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
The gene expression analysis cost more than expected. The experiments following the analysis of gene expression analysis needed extra expenditure. Thus, we have to wait a bit for next stage experiments.
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| 今後の研究の推進方策 |
Analysis of differentially expressed noncoding genes in mesothelioma cell lines using gene expression manipulation and its effect in chemotherapeutic agents.
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