| 研究課題/領域番号 |
22K07025
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| 研究種目 |
基盤研究(C)
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| 配分区分 | 基金 |
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| 応募区分 | 一般 |
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| 審査区分 |
小区分49030:実験病理学関連
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| 研究機関 | 九州大学 |
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研究代表者 |
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| 研究期間 (年度) |
2022-04-01 – 2025-03-31
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| 研究課題ステータス |
交付 (2022年度)
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| 配分額 *注記 |
4,160千円 (直接経費: 3,200千円、間接経費: 960千円)
2024年度: 1,170千円 (直接経費: 900千円、間接経費: 270千円)
2023年度: 910千円 (直接経費: 700千円、間接経費: 210千円)
2022年度: 2,080千円 (直接経費: 1,600千円、間接経費: 480千円)
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| キーワード | Anti-oxidant / Facial malformation / Zebrafish / Cell / omics |
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| 研究開始時の研究の概要 |
Treacher Collins syndrome (TCS) affects facial appearance and causes significant morbidity in facial form and function. It is necessary to identify the origin of the disease and the mechanisms mediating disease pathogenesis in order to treat the disease during the early developmental stages. The current hypothesis of applying the anti-oxidant during the embryo development may rescue the facial malformation, and we aim to unfold the molecular mechanism of such hypothesis in the TCS zebrafish model.
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| 研究実績の概要 |
The project applied the polr1c zebrafish mutant to study the Tracher Collins syndrome (TCS). Excessive reactive oxidative species were found in the mutant, and we have screened several anti-oxidants for potential treatment. In the past year, we have customized the facial micro-CT analysis in zebrafish for bone mineral density measurement (Liao et al. 2022). Besides, we analyzed the genotype-phenotype correlations in TCS (Ulhaq et al. 2022) and Nager syndrome (Ulhaq et al. In Press). Furthermore, we applied the developed methodologies to study the motor deficits in zebrafish (Ulhaq et al. 2023). Lastly, a review paper on deubiquitinates has been published (Liu et al. 2022).
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
We have explored several different areas about the TCS, from the micro-CT analysis, to the systematic review. Five SCI papers have been published in the past year. The planned biological experiments are on-going smoothly, including the dosage selection for resecure experiment. We are expecting to have some fruitful result by the end of the project.
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| 今後の研究の推進方策 |
The research will be performed as scheduled. Briefly, resecure experiments will be done with the imaging, real-time qPCR, and other biochemistry assays. RNA-sequencing on the enriched neural crest cells will be performed as well.
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