| 研究課題/領域番号 |
22K07320
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| 研究種目 |
基盤研究(C)
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| 配分区分 | 基金 |
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| 応募区分 | 一般 |
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| 審査区分 |
小区分51010:基盤脳科学関連
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| 研究機関 | 富山大学 |
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研究代表者 |
YOUNG MEGAN 富山大学, 学術研究部医学系, 特命助教 (50930830)
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| 研究分担者 |
田村 了以 富山大学, 学術研究部医学系, 教授 (60227296)
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| 研究期間 (年度) |
2022-04-01 – 2025-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
4,160千円 (直接経費: 3,200千円、間接経費: 960千円)
2024年度: 1,040千円 (直接経費: 800千円、間接経費: 240千円)
2023年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
2022年度: 1,820千円 (直接経費: 1,400千円、間接経費: 420千円)
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| キーワード | hippocampus / non-human primate / monkey / CA1 / place field / freely-moving / neuronal recording / primate / electrophysiology |
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| 研究開始時の研究の概要 |
The firing patterns of spatially-selective neurons in the hippocampus play a critical role in spatial navigation. Using a 3D maze, a wireless data logger, and deep learning-powered pose estimation, we will compare the horizontal and vertical response properties of place cells in the hippocampus.
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| 研究実績の概要 |
Our purpose in this study is to record neuronal activity from the hippocampus of freely-moving monkeys in a three-dimensional environment to evaluate the relationship between movement direction and place cell responsiveness. We have trained a monkey to move within this three-dimensional environment by rotating clockwise or counterclockwise, and continue development of a primate behavior monitoring system using AI. This year, we used evoked potentials to accurately map the location of the hippocampus and successfully recorded a number of hippocampal neurons stably with good signal within our three-dimensional environment under awake conditions using telemetry.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Behavioral monitoring using a camera system with TTL synchronization to the neuronal recording via telemetry has been established and works reliably. The monkey has been well trained to navigate the 3D environment in both the clockwise and counter clockwise directions. Mapping to locate the hippocampus with high fidelity has been performed and has enabled precise targeting of the microdrive-equipped tetrodes. Neuronal recording via chronically implanted tetrodes has also been proceeding smoothly, with stable signal over long time courses, including during behavior as the animal freely navigates the 3D environment.
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| 今後の研究の推進方策 |
We will continue to record signals from more hippocampal neurons as the monkey navigates the 3D environment, with simultaneous synchronized video tracking using our camera system. We will proceed with analyzing the recorded signals with cluster cutting and analysis of the spike data using the animal positions provided by our behavioral tracking system.
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