| 研究課題/領域番号 |
22K12408
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| 研究種目 |
基盤研究(C)
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| 配分区分 | 基金 |
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| 応募区分 | 一般 |
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| 審査区分 |
小区分63040:環境影響評価関連
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| 研究機関 | 国立研究開発法人国立環境研究所 |
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研究代表者 |
Tin・Tin Win・Shwe 国立研究開発法人国立環境研究所, 環境リスク・健康領域, シニア研究員 (00391128)
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| 研究期間 (年度) |
2022-04-01 – 2025-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
4,160千円 (直接経費: 3,200千円、間接経費: 960千円)
2024年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
2023年度: 1,170千円 (直接経費: 900千円、間接経費: 270千円)
2022年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円)
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| キーワード | microplastics / nanoplastics / combined exposure / carbon nanoparticles / neurotoxicity / rats / environmental pollutants / single/combined exposure / neurobehavior / Microplastics / Environmental pollutants / Single/combined exposure / Neurotoxicity / Neurobehavior |
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| 研究開始時の研究の概要 |
Microplastics (MPs) pollution has gradually become into a global environmental problem and paid attention by government, society and academia. However, it is unclear whether exposure to MPs affects living organisms, can co-exposure with other environmental pollutants affects central nervous system and early life exposure causes developmental neurotoxicity in later life. Understanding the effects and mechanism of MPs exposure and combined exposure with well-known environmental pollutants would be helpful for neurotoxicity assessment, environmental management, and environmental conservation.
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| 研究実績の概要 |
The rats were divided into four groups: 1) control group, 2) polystyrene nanoplastics (PSNP) group (50 mg/Kg, 3 days/week, 4 weeks, oral), 3) carbon nanoparticles (CN) group (20 μg/m3 5 h/day, 5 days/week, 4 weeks, inhalation), and 4) PSNP + CN group. PSNP, CN or PSNP+CN-groups showed a significant reduction in the number of center entries and duration of stay. PSNP alone-exposed rats showed poor discrimination ability. Increased glutamate levels, and decreased NMDA subunits (NR1, NR2B), and CREB1, CAMKIV mRNA were observed in the hippocampus of CN alone or PSNP + CN groups. SOD and CAT were significantly lower, while IL-1beta, TNF-alpha and Iba1 mRNAs were remarkably higher in CN alone or PSNP+CG groups. Thus, combined exposure to PSNP and CN had synergistic toxic effects.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
As a research plan, the experiments were performed, and the results were presented in domestic (Japan society of Hygiene) and international (European Society of Toxicology) conferences.
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| 今後の研究の推進方策 |
Pregnant rats (n = 24) will be allocated to four groups as 1) the control group, 2) PSNP oral exposure group (50 mg/Kg, 3 days/week, 4 weeks), 3) carbon nanoparticles (CN, 20 μg/m3 5 h/day, 5 days/week, 4 weeks) inhalation exposure group and 4) PSNP + CN exposure group. PSNP group will be administered PSNP dissolved in sterile water from gestational (GD) 14 to postnatal day (PND) 21 in the clean air and CN chamber. At the age of 9-week-old, male and female offspring (n = 8) will be subjected to cognitive and anxiety behaviors. Then, neurological, and immunological biomarkers in the prefrontal cortex and hippocampus of male and female rats will be examined using real time RT-PCR and immunohistochemical analyses.
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