| 研究課題/領域番号 |
22K12428
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| 研究種目 |
基盤研究(C)
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| 配分区分 | 基金 |
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| 応募区分 | 一般 |
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| 審査区分 |
小区分64020:環境負荷低減技術および保全修復技術関連
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| 研究機関 | 大阪大学 |
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研究代表者 |
Luong Van・Duc 大阪大学, 大学院工学研究科, 特任研究員(常勤) (60815174)
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| 研究分担者 |
井上 大介 大阪大学, 大学院工学研究科, 准教授 (70448091)
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| 研究期間 (年度) |
2022-04-01 – 2025-03-31
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| 研究課題ステータス |
交付 (2022年度)
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| 配分額 *注記 |
4,160千円 (直接経費: 3,200千円、間接経費: 960千円)
2024年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2023年度: 1,170千円 (直接経費: 900千円、間接経費: 270千円)
2022年度: 1,560千円 (直接経費: 1,200千円、間接経費: 360千円)
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| キーワード | anaerobic digestion / high ammonium / propionate / propionate oxidizers / carbon recovery / methane recovery / propionate oxidizer / methane producer |
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| 研究開始時の研究の概要 |
Anaerobic digestion (AD) is a sustainable technology for CH4 recovery from wastewater as energy. Yet, the application of AD under high NH4+ remains a big challenge because high NH4+ inhibits the activity of propionate-oxidizing bacteria (POB), which results in low production of CH4. We will enrich and couple NH4-tolerant POB with methane-producing archaea for the enhanced CH4 recovery under high NH4+.
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| 研究実績の概要 |
We have successfully enriched NH4-tolerant propionate-oxidizing bacteria by batch transfers. The enrichment culture can oxidize propionate under 5 gNH4-N/L. Next-generation sequencing showed the dominance of propionate-oxidizing bacteria, including Pelotomaculum and Cryptanaerobacter. In addition, the increase of hydrogenotrophic methanogens Methanoculleus was also observed, indicating the syntrophic relationship between NH4-tolerant propionate-oxidizing bacteria and hydrogenotrophic methanogens.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
Because the activity of NH4-tolerant propionate-oxidizing bacteria is unstable after several transfers. Therefore, the enrichment period is extended more than expected to optimize the enrichment culture and increase biomass. The extended period is necessary to maintain the activity of propionate-oxidizing bacteria before proceeding to the following steps.
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| 今後の研究の推進方策 |
Higher concentrations of propionate and NH4+ will be introduced to test the tolerance of the enriched culture. Next, different combinations of propionate-oxidizing bacteria and archaea will be considered for stable and effective production of CH4 under high NH4+ concentrations.
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