| 研究課題/領域番号 |
22K14711
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分34020:分析化学関連
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| 研究機関 | 国立研究開発法人物質・材料研究機構 |
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研究代表者 |
GANGANBOINA AkhileshBabu 国立研究開発法人物質・材料研究機構, 若手国際研究センター, ICYS研究員 (90937252)
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| 研究期間 (年度) |
2022-04-01 – 2024-03-31
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| 研究課題ステータス |
交付 (2022年度)
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| 配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2023年度: 2,080千円 (直接経費: 1,600千円、間接経費: 480千円)
2022年度: 2,600千円 (直接経費: 2,000千円、間接経費: 600千円)
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| キーワード | Virus sensor / Photoelectrochemical / self powered / Nanohetrostructure / Biosensor / Virus / Self powered / Nanomaterial |
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| 研究開始時の研究の概要 |
In this project, we will focus on the developing a cost-effective self-powered photoelectrochemical virus biosensing platform for the rapid point of care diagnosis. A completely new attempt for the visible-light-driven self-powered device based on Co3O4-CdSe-CdS-Pt NPs will be developed for real-time detection. The detection signal is majorly based on the photocurrent response generated by Co3O4-CdSe-CdS-Pt upon excitation with visible light. The whole project's success can make a self-powered visible-light-driven device for a rapid detection of viruses.
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| 研究実績の概要 |
Self-powered photoelectrochemical (PEC) sensors have recently attracted a lot of attention in healthcare systems. PEC detection inherits the benefits of both electrochemical and optical techniques as the photodriven electrochemical reaction is fundamental to PEC analysis, making it necessary to increase the associated PEC signals for sensitive detection. Investigations into the production, separation, and interfacial-redox-reaction mechanisms of photoinduced carriers are crucial for the realization of effective PEC detection at present. However, the slow interfacial interactions of the photogenerated carriers, as well as the necessity for suitable photoactive layers for sensing, continue to pose obstacles to the development of sophisticated PEC platforms. The important achievement in this work is well-defined novel architecture of Co3O4-CdSe-CdS-Pt using hollow sphere, rod and quantum dot allowing to adjust Pt and Co3O4 position at a particular distance regulates the hole and electron recombination rate, improving the photo-catalytic performance. The detection signal is majorly based on the photo current response generated by novel electrocatalyst Co3O4-CdSe-CdS-Pt upon excitation with visible light. For the amplification of the detection signal, Co3O4-CdSe-CdS-Pt will be encapsulated inside the liposome, functionalized with antibodies. Conjugating the Co3O4-CdSe-CdS-Pt encapsulated liposomes with virus, followed by magnetic separation allows the variation of analytical signal in response to virus concentration.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Synthesis of Co3O4-CdSe-CdS-Pt nanocomposite.
Sequential formation of nano heterostructure (Co3O4-CdSe-CdS-Pt) with increasing complexity comprised of four stages. The synthesis steps begin with forming cobalt oxide nanoparticles (Co3O4 NPs) by decomposition of cobalt nitrate with benzylamine in the presence of ammonium hydroxide. Then, the CdSe QD was grown on the surface of cobalt oxide nanoparticles. The cobalt oxide-CdSe complex synthesized serve as a seed for the CdS rod growth. In the dimer CdSe serve as nucleation site for the rod growth, and the rod grew exclusively on CdSe portion of the dimer leaving Cobalt oxide one end of the rod. Finally, the rods were tipped with Pt nanoparticle on another end of the rod.
To date, this is the first report synthesizing the nano heterostructure, Co3O4-CdSe-CdS-Pt for PEC based virus detection. We were also successful in encapsulation of the prepared Co3O4-CdSe-CdS-Pt NPs in to liposomes for the signal amplification
Currently, most of the efforts are focused on the synthesis, optimizing the growth conditions, understanding the chemical reactions, and improving reproducibility. Based on the gained knowledge on synthesis of Co3O4-CdSe-CdS-Pt, optimizing the distance between Co3O4 and Pt NPs which play an important role in interfacial-redox-reaction mechanisms increased substantially.
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| 今後の研究の推進方策 |
Synthesis of wafer-scale 2D TMDC single crystals.
Beside the successful synthesis of Co3O4-CdSe-CdS-Pt, it is also important to evaluate the quality and uniformity of the prepared nanoparticles. More characterization will be employed to make sure there is uniformity in particle size. More PEC electrodes will be fabricated and tested. With the photo current intensity and uniformity information, growth conditions will be further optimized.
Sensor preparation and its detection measurements:
The antibody-conjugated Co3O4-CdSe-CdS-Pt NPs -embedded liposome NVs and Ab-Fe3O4 will be mixed with different concentration of viruses, allowing formation of sandwich-structured immunocomplex. The exact ratio of each precursor with the optimum condition to get the best suited immunocomplexes can be optimized carefully. The formed immunocomplex will be isolated from the sample matrix by simple magnetic separation. Using surfactants, the separated liposome NVs can be melted and release several Co3O4-CdSe-CdS-Pt NPs. The released Co3O4-CdSe-CdS-Pt NPs act as photocatalyst and are deposited on to electrode. Thus, prepared electrode is placed in to the electrolyte chamber and measure the photo current.
After the optimization of synthesis and detection prameters, we will also perform the detection of virus in real samples.
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