| 研究課題/領域番号 |
22K14982
|
|---|
| 研究種目 |
若手研究
|
| 配分区分 | 基金 |
|---|
| 審査区分 |
小区分42010:動物生産科学関連
|
|---|
| 研究機関 | 東北大学 |
|---|
研究代表者 |
Binghui Zhou 東北大学, 農学研究科, 助教 (70906031)
|
|---|
| 研究期間 (年度) |
2022-04-01 – 2024-03-31
|
| 研究課題ステータス |
完了 (2023年度)
|
| 配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2023年度: 2,470千円 (直接経費: 1,900千円、間接経費: 570千円)
2022年度: 2,210千円 (直接経費: 1,700千円、間接経費: 510千円)
|
|---|
| キーワード | wakame assimilation / immunobiotic / immunosynbiotic / porcine / immune response / Immunobiotics / Immunosynbiotics / Wakame / Lactobacilli / Porcine intestinal cell / Tolerance to acid / Porcine / Probiotics / Immunogenics |
|---|
| 研究開始時の研究の概要 |
In order to minimize the impact of antibiotic resistance in livestock production, immunosynbiotics have been proposed to be candidates for antibiotic substitutes referring to immunomodulatory microorganisms (immunobiotics) and wakame (immunoprebiotics). I aim to determine sugar utilization and host immune modulatory patterns of selected immunobiotic strains using biochemical, genomic and immunological approach. Finally, the efficacy of the immunosynbiotics will be evaluated using in vitro and in vivo porcine models to improve the healthy growth of antibiotic-independent porcine production.
|
| 研究実績の概要 |
Antibiotic resistance caused by the overuse of antibiotics in livestock production has been a severe problem, leading to the need for effective alternatives. A promising alternative to antibiotics is the "immunosynbiotics," a combination of immunobiotics and immunoprebiotics. The seaweed wakame is considered an immunoprebiotic, and L. salivarius is an immunobiotic, isolated from the intestine of wakame-fed pigs. Adding wakame to pig feed has been found to improve the immune response and alter the microbiota in the porcine large intestine. Using wakame as a feed supplement could also provide an economic recovery alternative in Miyagi prefecture after the 2011 Tohoku earthquake. These results were achieved in this study:
(1) Eight lactobacilli strains thrived better in wakame-based broths with added B vitamins, showcasing varying wakame utilization abilities based on genomic analysis. (2) A strain with adhesion to porcine intestinal epithelial cells survived simulated gastric juice with wakame due to its fiber content shielding cells from acidic conditions, facilitating potential probiotic effects. (3) We conducted an in vivo study by feeding 36 piglets with wakame fermentation using this promising strain. Furthermore, our observations demonstrated that the abundance of beneficial Lactobacillus bacteria increased, while pathogenic strains such as E. coli decreased.
The results indicate that the potential applications of immunosynbiotics in promoting overall well-being are exciting areas for further investigation and could lead to practical ways to enhance health in the future.
|
