| 研究課題/領域番号 |
22K15159
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分44050:動物生理化学、生理学および行動学関連
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| 研究機関 | 名古屋大学 |
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研究代表者 |
SU Matthew 名古屋大学, 高等研究院(理), 特任助教 (00885981)
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| 研究期間 (年度) |
2022-04-01 – 2025-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
4,550千円 (直接経費: 3,500千円、間接経費: 1,050千円)
2024年度: 1,040千円 (直接経費: 800千円、間接経費: 240千円)
2023年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2022年度: 2,080千円 (直接経費: 1,600千円、間接経費: 480千円)
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| キーワード | Mosquito / Hearing / Ionotropic receptor / Acoustic behavior / Electrophysiology / Aedes mosquitoes / Thermosensation / Ionotropic receptors / Ionotropic Receptors |
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| 研究開始時の研究の概要 |
Male mosquitoes identify females by listening for their flight sounds. These sounds change with temperature, yet males can still locate females. This project will improve our understanding of how male hearing changes with temperature and suggest new methods of disrupting hearing to prevent mating.
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| 研究実績の概要 |
Working with national and international collaborators, I investigated the role played by two ionotropic receptors (IR21a and IR25a) in influencing mosquito hearing function behaviors. Working with colleagues at the ITbM at Nagoya University, I conducted transcriptomic and proteomic analyses of different mosquito tissues to identify expression patterns of these (and other receptors). Working with collaborators at the NHRI, Taiwan, I generated a knockout mutant for one of these receptors (IR21a) as well as several antibodies for western blotting. Finally, working in Nagoya, I tested the effect of gene knockout on mosquito hearing function using novel assays. Though IR21s appears expressed in the ear, I found no effect of IR21a knockout on hearing function.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
I have successfully generated one of the two mutants I originally planned to target, although gene knockout does not seem to influence hearing function. I am currently working to generate the other knockout mutant and believe it should be completed in several months. My transcriptomic and proteomic screens of different tissues identified many more IRs that could be the target of further research, broadening the scope of my project. Via national and international collaborations, I was able to interact with new researchers and discuss novel ideas for future projects.
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| 今後の研究の推進方策 |
This year I will complete generation of the other knockout mutant (IR25a) and test the effect of this knockout on hearing function and behavior in different environments. I will also look to generate further IR knockout mutants and IR antibodies with my international collaborators to test if they influence hearing function. My experimental pipeline is now streamlined and I believe these experiments should be completed promptly.
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