| 研究課題/領域番号 |
22K15234
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分46030:神経機能学関連
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| 研究機関 | 国立研究開発法人理化学研究所 |
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研究代表者 |
Guan Hefei 国立研究開発法人理化学研究所, 脳神経科学研究センター, 特別研究員 (00930273)
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| 研究期間 (年度) |
2022-04-01 – 2025-03-31
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| 研究課題ステータス |
交付 (2022年度)
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| 配分額 *注記 |
4,420千円 (直接経費: 3,400千円、間接経費: 1,020千円)
2024年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2023年度: 1,040千円 (直接経費: 800千円、間接経費: 240千円)
2022年度: 1,950千円 (直接経費: 1,500千円、間接経費: 450千円)
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| キーワード | Spatial memory / Hippocampus / Lateralization / Memory mechanisms |
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| 研究開始時の研究の概要 |
I'll test whether left and right CA3 play distinct and complementary roles in memory which will be a significant step forward in the field, demonstrating that functional lateralization in the hippocampal circuit is evolutionary conserved across rodents and humans.
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| 研究実績の概要 |
The first stage of the project involved characterizing the inhibitory effects and time course of viral expression of Gi-DREADD and KOR-DREADD in the hippocampus, as well as examining their corresponding ligand effects on in vivo hippocampal activity.
For the second stage, behavioral tasks were conducted to investigate the different functions of the left and right CA3 regions in spatial navigation and memory. In the object location test, mice were trained with two familiar objects, and during the second session of recall, one of the objects was moved to a different location after the left CA3 was silenced instead of the right CA3. The mice spent more time exploring the object in the novel location, which is attributed to their innate preference for novelty. These findings suggest that the left CA3 region is more crucial for the acquisition of spatial memory.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The research project is progressing relatively smoothly. The planned assessment of DREADD-mediated silencing of neural activity in the bilateral hippocampus has been completed as scheduled, as have the behavioral tasks designed to test the spatial navigation and memory functions of the left and right CA3 regions. However, there has been a delay in starting the in vivo recording experiment due to the late delivery of a camera necessary for detecting animal behavior in the object location test. We are expecting to receive the new camera soon, and to ensure that we stay on track with the planned schedule, a part-time student has been hired to assist with the preparation of the recording experiment.
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| 今後の研究の推進方策 |
As outlined in the grant proposal, the first step of the study will be to investigate whether the output of the left and right CA3 regions plays differential roles in the coordination of bilateral CA1 spatial coding. This will be achieved by injecting different viruses in the left and right CA3 regions and recording simultaneously in the bilateral CA1 regions while mice navigate a linear track.
Additionally, the study will involve recording from bilateral CA1 regions while mice perform object location tasks, in order to further understand the mechanisms underlying the differences between the left and right CA3 regions in spatial memory. I will adhere to the plan outlined in the grant proposal throughout the study.
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