| 研究課題/領域番号 |
22K15532
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分50010:腫瘍生物学関連
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| 研究機関 | 藤田医科大学 |
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研究代表者 |
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| 研究期間 (年度) |
2022-04-01 – 2025-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
4,550千円 (直接経費: 3,500千円、間接経費: 1,050千円)
2024年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円)
2023年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2022年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
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| キーワード | Adipsin / Breast cancer / Adipokine / Invasion / Adipocyte / Cancer stem cell |
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| 研究開始時の研究の概要 |
Adipsin is mainly secreted from adipocytes and enhances the cancer stem cell (CSC) properties of breast cancer cells.
Adipsin secretion is dependent on adipocyte maturation.
Here, we investigate molecular mechanisms by which Adipsin regulates adipocyte maturation to promote adipocyte-CSC interactions.
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| 研究実績の概要 |
We investigated the role of mature adipocytes in breast tumor migration and invasion compared to adipocyte progenitor cells.
1.Mature adipocytes had a significantly higher capacity to induce breast tumor migration and invasion than adipocyte progenitor cells. Hepatocyte growth factor (HGF) restored the reduced invasion-promoting ability of Complement Factor D knockout (Cfd-KO) mature adipocytes.
2.In cancer cells co-cultured with mature adipocytes, invasion promoters galectin-7 (Lgals7) and matrix metalloproteinases (MMPs) were upregulated.
3.Utilizing a syngeneic mouse model, we observed that tumor growth and metastasis were significantly suppressed in Adipsin-KO mice, with reduced capsular formation, tumor invasion at the cancer-adipocyte interface, and distant metastasis.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
We are feeding mice a high-fat diet to investigate the effect of Adipsin on regulating body weight. The first round of the experiment has been completed, and we are currently conducting the second round of in vivo experiments. However, the food intake amounts of the mice differ between the two experiments, causing some inconsistencies.
We are examining whether Adipsin-KO mice fed a high-fat diet can inhibit the growth of breast cancer tumors. Additionally, we are investigating whether high-fat diet-fed Adipsin-KO mice injected with breast cancer cells exhibit different immune cell infiltration in the tumor lesion.
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| 今後の研究の推進方策 |
We will address inconsistencies in mice's food intake during high-fat diet experiments by standardizing feeding protocols. Future work includes expanding our study to additional cohorts of Adipsin-KO and wild-type mice to investigate Adipsin's role in body weight regulation and breast cancer growth. We will also explore the molecular mechanisms underlying immune cell infiltration differences in tumor lesions of high-fat diet-fed Adipsin-KO mice.
Challenges such as variability in food intake and stress factors will be mitigated by controlling environmental conditions and maintaining consistent feeding schedules. We will employ rigorous randomization and blinding techniques to enhance the reliability of our results.
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