| 研究課題/領域番号 |
22K15656
|
|---|
| 研究種目 |
若手研究
|
| 配分区分 | 基金 |
|---|
| 審査区分 |
小区分51030:病態神経科学関連
|
|---|
| 研究機関 | 兵庫医科大学 |
|---|
研究代表者 |
段 ショウキ 兵庫医科大学, 医学部, 助教 (20910607)
|
|---|
| 研究期間 (年度) |
2022-04-01 – 2025-03-31
|
| 研究課題ステータス |
交付 (2023年度)
|
| 配分額 *注記 |
4,550千円 (直接経費: 3,500千円、間接経費: 1,050千円)
2024年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円)
2023年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2022年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
|
|---|
| キーワード | SNS / IBS / Micro-inflammation / Visceral pain / sympathetic nervous / eosinophil |
|---|
| 研究開始時の研究の概要 |
Patients who suffer from irritable bowel syndrome often exhibit abnormal psychological profiles like depression, which is closely related to the sympathetic nervous system (SNS) activity. SNS-immune interaction is considered important to chronic disease development. Recently, we successfully established a maternal separation-induced IBS animal model, which showed increased activity of SNS and micro-inflammation in the gastrointestinal (GI) tract. The proposed project aimed to investigate whether and how SNS modulates eosinophil in the GI tract, and their involvement in pathophysiology of IBS.
|
| 研究実績の概要 |
This year, I primarily conducted genetic modulation experiments. Using DBH-cre mice and AAV-PHP.s-Gq, I successfully activated sympathetic nervous system (SNS) activity through CNO injection, as confirmed by double immunohistochemical staining of cre and mcherry, and the NE concentration in the serum. The results indicated that immune cells were recruited into the GI tract following SNS activation, consistent with findings in the MS model. As for the underlying mechanisms, I checked several cytokines and chemokines, and aims to clarify the connection between SNS activity and immune alteration.
I attended two academic conferences this year. I did symposium speaking in the 46th Annual Meeting of the Japan Neuroscience Society. And I did an oral presentation in the 45th Annual Meeting of Japanese Association for the Study of Pain.
|
| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
I proved that activate SNS recruited immune cells into the GI tract using genetic modulation method. By those data, I achieved the second step of the project.
|
| 今後の研究の推進方策 |
In the next year, I will clarify the underlying mechanism that SNS modulates the immune cell immigration. Especially the cytokines, chemokines and related cell type. In addition, I will summarize the data and prepare the manuscript to publish the article.
|
