| 研究課題/領域番号 |
22K15656
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分51030:病態神経科学関連
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| 研究機関 | 兵庫医科大学 |
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研究代表者 |
段 ショウキ 兵庫医科大学, 薬学部, 博士研究員 (20910607)
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| 研究期間 (年度) |
2022-04-01 – 2025-03-31
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| 研究課題ステータス |
交付 (2022年度)
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| 配分額 *注記 |
4,550千円 (直接経費: 3,500千円、間接経費: 1,050千円)
2024年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円)
2023年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2022年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
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| キーワード | SNS / IBS / Micro-inflammation / Visceral pain / sympathetic nervous / eosinophil |
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| 研究開始時の研究の概要 |
Patients who suffer from irritable bowel syndrome often exhibit abnormal psychological profiles like depression, which is closely related to the sympathetic nervous system (SNS) activity. SNS-immune interaction is considered important to chronic disease development. Recently, we successfully established a maternal separation-induced IBS animal model, which showed increased activity of SNS and micro-inflammation in the gastrointestinal (GI) tract. The proposed project aimed to investigate whether and how SNS modulates eosinophil in the GI tract, and their involvement in pathophysiology of IBS.
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| 研究実績の概要 |
This research aims to investigate the relationship and potential mechanism between sympathetic nervous system (SNS) and micro-inflammation. In this year, I established the maternal separation (MS) model to mimic IBS. This model showed visceral hypersensitivity to colorectal distention, which is the main pathophysiology of IBS. I confirmed that there is micro-inflammation in colon of MS rats at adulthood by flowcytometry method and IHC. Besides, I researched the SNS activity. The ELISA result showed that NE concentration in serum increased in the MS rats compared with control. Neural activation markers are higher in MS model rats compared with control. Taken together, I confirmed that MS rats showed over activation of SNS. In addition, I applied the 6-OHDA to degenerate the SNS, the pharmacological data suggested that SNS played a role in immune cell alteration in colon. I also started to establish the genetic modulation approach to specifically modulation SNS activity.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
In this year, I established the IBS model with micro-inflammation and over activation of SNS by MS stress. I pharmacologically confirmed that SNS activation modulate immune alteration in colon. By those data, I achieved the first step of the project.
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| 今後の研究の推進方策 |
In the next year, I will focus on the genetic modulation of peripheral SNS by DBH-cre mice with AAV-PHP.S infection. Using this method, I will specifically activate or inhibit the peripheral SNS and check the immune alteration.
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