研究課題/領域番号 |
22K16033
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研究種目 |
若手研究
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配分区分 | 基金 |
審査区分 |
小区分53010:消化器内科学関連
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研究機関 | 国立感染症研究所 |
研究代表者 |
闕 路晟 国立感染症研究所, ウイルス第二部, 研究員 (20941636)
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研究期間 (年度) |
2022-04-01 – 2025-03-31
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研究課題ステータス |
交付 (2022年度)
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配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2024年度: 1,820千円 (直接経費: 1,400千円、間接経費: 420千円)
2023年度: 1,560千円 (直接経費: 1,200千円、間接経費: 360千円)
2022年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
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キーワード | HBV / Ferroptosis / infected / clearance / ferroptosis |
研究開始時の研究の概要 |
Hepatitis B virus is a causative agent of serious liver illness. Current antiviral drugs have failed to eradicate infected cells. Focusing on a novel type of cell death, the mechanism how HBV and host immune response sensitize the infected cells to ferroptosis will be investigated.
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研究実績の概要 |
The Hepatitis B virus is a causative agent of Chronic hepatitis B, liver cirrhosis, and hepatocellular carcinoma. Current antiviral drugs interfering viral life cycle, have failed to eradicate infected cells, and the strategies to selectively eliminate infected cells have been awaited. Focusing on a novel type of cell death, the mechanism of how HBV and host immune response sensitize the infected cells to ferroptosis will be investigated.
This year, we found a couple of anti/pro-ferroptotic genes expression is differentially expressed between no-HBV&HBV&HBV+anti-viral drug-treated in a dry/wet analysis; When a human hepatocyte cell line HepG2-NTCP was infected with HBV, a ferroptosis agonist and antagonist decreased and increased HBV infection by in-vitro experiment, respectively;
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現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
We can spend more time in the laboratory to carry forward this project this year compared with one year ago during the SARS-CoV-2 pandemic.
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今後の研究の推進方策 |
We have currently identified a couple of anti/pro-ferroptotic genes which involved in HBV infection. In the next year, we try to clarify the role of those genes in HBV infection by performing a lost/gain of function approach. The effect of these genes on viral markers and cell viability will be quantified.
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