| 研究課題/領域番号 |
22K16033
|
|---|
| 研究種目 |
若手研究
|
| 配分区分 | 基金 |
|---|
| 審査区分 |
小区分53010:消化器内科学関連
|
|---|
| 研究機関 | 国立感染症研究所 |
|---|
研究代表者 |
闕 路晟 国立感染症研究所, ウイルス第二部, 研究員 (20941636)
|
|---|
| 研究期間 (年度) |
2022-04-01 – 2025-03-31
|
| 研究課題ステータス |
交付 (2023年度)
|
| 配分額 *注記 |
4,680千円 (直接経費: 3,600千円、間接経費: 1,080千円)
2024年度: 1,820千円 (直接経費: 1,400千円、間接経費: 420千円)
2023年度: 1,560千円 (直接経費: 1,200千円、間接経費: 360千円)
2022年度: 1,300千円 (直接経費: 1,000千円、間接経費: 300千円)
|
|---|
| キーワード | HBV / Ferroptosis / infected / clearance / ferroptosis |
|---|
| 研究開始時の研究の概要 |
Hepatitis B virus is a causative agent of serious liver illness. Current antiviral drugs have failed to eradicate infected cells. Focusing on a novel type of cell death, the mechanism how HBV and host immune response sensitize the infected cells to ferroptosis will be investigated.
|
| 研究実績の概要 |
We discovered that HBV infection can influence the expression of certain genes related to ferroptosis in both HBV-infected and uninfected groups. In 2023, our goal is to explore the role of these genes in HBV infection. Using a loss-of-function approach with siRNA, we observed that reducing a ferroptosis biomarker in an HBV-infected cell line (NTCP-HepG2) led to the following results: 1) a slight increase in cell viability; 2) a decrease in HBV markers such as HBV DNA, HBsAg, and HBeAg. Subsequently, we used an inhibitor targeting a ferroptosis biomarker to validate the siRNA results, which showed similar trends. Additionally, the phenotypic detection of ferroptosis indicated that the siRNA-transfected group exhibited a significant decrease in lipid ROS compared to the NC group.
|
| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
As indicated in the research summary, progress for the second year is generally on track.
|
| 今後の研究の推進方策 |
In 2024, our goal is to comprehend the function of this gene in living organisms. To achieve this, we will use the hydrodynamic injection (HDI) HBV replication mice model. We will deliver either the siRNA or the inhibitor of the target gene. Subsequently, we will quantify markers of ferroptosis and HBV infection to the HDI HBV to gain a better understanding of the role of this gene.
|
