| 研究課題/領域番号 |
22K17010
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| 研究種目 |
若手研究
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| 配分区分 | 基金 |
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| 審査区分 |
小区分57020:病態系口腔科学関連
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| 研究機関 | 北海道大学 |
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研究代表者 |
ハビバ ウンマ 北海道大学, 医学研究院, 客員研究員 (40755914)
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| 研究期間 (年度) |
2022-04-01 – 2025-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
4,550千円 (直接経費: 3,500千円、間接経費: 1,050千円)
2024年度: 1,170千円 (直接経費: 900千円、間接経費: 270千円)
2023年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2022年度: 1,950千円 (直接経費: 1,500千円、間接経費: 450千円)
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| キーワード | cancer stem cells (CSCs) / DN gels / drug-resistant / keyworad / cancer stem cells / head and neck cancer / hydrogel / reprogramming |
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| 研究開始時の研究の概要 |
Cancer recurrence can arise due to rare circulating cancer stem cells (CSCs) resistant to chemotherapies and radiotherapies. This research focuses on the induction of head and neck cancer stem cells (HNCSCs) using hydrogel as a biomaterial. This study will develop suitable methods for detecting HNCSCs to create a personalized therapeutic modality. The study aims are:
1. Investigation of the mechanism of HNCSCs induction by the hydrogel.
2. Development of a diagnostic method for HNCSCs.
3. Establishment of a drug screening system for clinical application.
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| 研究実績の概要 |
Cancer recurrence can arise due to circulating cancer stem cells (CSCs) resistant to treatment. This research focuses on inducing head and neck cancer stem cells (HNCSCs) using hydrogel as a biomaterial. The study aims to investigate the mechanism of HNCSCs induction by the hydrogel, develop a diagnostic method for HNCSCs, and establish a drug screening system for clinical application.
The proposed study will elucidate the mechanism of hydrogel-induced reprogramming of cancer cell populations to identify new CSC markers.
We characterized the hydrogel-induced sphere as forming head and neck SCC cells. We cultured 4 HNSCC cells on DN gels for 5 days and checked SOX-2, Nanog, OCt3/4, and ALDH1 expression for up to 5 days by PCR. CSC markers show elevated expression as time elapses.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
4: 遅れている
理由
Currently, I cannot do research as a full-time researcher. Due to my job responsibilities, current research progress is delayed. However, I could speed up my research from August 2024 onward.
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| 今後の研究の推進方策 |
2024-2025 Fiscal Year Plan:
(I) To elucidate the mechanism of induction of CSCs by hydrogel by analysis of mechanosignal molecules and by genome/epigenome analysis
(II) To develop a CSC diagnostic method using hydrogel by Identifying new CSC markers (Microarray and RNAseq analysis will be performed) and creating a personalized medical hydrogel kit using Patient-derived primary cultured cells to determine hydrogel conditions that can predict human tumors' recurrence profile after treatment.
(III) To create seeds for CSC therapy using hydrogel by developing a drug screening system and identifying seeds using a hydrogel plate, we will do the drug screening, intending to obtain drug seeds that can be applied to medical treatment by performing large-scale screening targeting CSCs.
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