研究課題/領域番号 |
22K17259
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研究種目 |
若手研究
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配分区分 | 基金 |
審査区分 |
小区分57070:成長および発育系歯学関連
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研究機関 | 北海道医療大学 |
研究代表者 |
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研究期間 (年度) |
2022-04-01 – 2024-03-31
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研究課題ステータス |
中途終了 (2022年度)
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配分額 *注記 |
4,550千円 (直接経費: 3,500千円、間接経費: 1,050千円)
2024年度: 1,690千円 (直接経費: 1,300千円、間接経費: 390千円)
2023年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2022年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
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キーワード | Amleogenin / Clone / Malassez / malassez / amelogenin |
研究開始時の研究の概要 |
The importance of these three trials is that they establish a therapeutic strategy for the regeneration of the entire PDL (tissue around tooth) complex to restore the functions of the tooth. Combining these three phases could help develop a therapeutic strategy for the regeneration of hard tissue or PDL tissue, from basic research to clinical application.
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研究実績の概要 |
Epithelial cell rests of Malassez (ERM) are an easy source of stem cell-like cells. Our previous work has successfully demonstrated that an ERM variant (isolated clone of ERM) can maintain healthy periodontal space by inhibiting ankylosis between the tooth and alveolar bone. This study aims to use the applicant's previously isolated clone model to repair the lost or injured periodontal ligament (PDL) tissue to restore tooth form and function. Phase-1: We obtained primary ERM from PDL tissues of porcine and isolated clones of ERM. We isolated clones 1-10. The characterization was done using immunofluorescence staining of ERM cell markers.
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現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
We have already isolated clones and characterized their origin as Epithelial cell rests of malassez markers.
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今後の研究の推進方策 |
Phase-2: ERM clones will be checked for enamel matrix proteins and some stem cell markers. Pilot experiments to combine some cells to produce cell sheets. Phase-3: Histological study of the transplanted tooth. Next-generation sequencing of ERM clones with stem cell-like properties and analysis to identify essential genes.
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