研究課題/領域番号 |
22K18179
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研究種目 |
若手研究
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配分区分 | 基金 |
審査区分 |
小区分90110:生体医工学関連
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研究機関 | 北海道大学 |
研究代表者 |
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研究期間 (年度) |
2022-04-01 – 2024-03-31
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研究課題ステータス |
交付 (2022年度)
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配分額 *注記 |
4,550千円 (直接経費: 3,500千円、間接経費: 1,050千円)
2023年度: 910千円 (直接経費: 700千円、間接経費: 210千円)
2022年度: 3,640千円 (直接経費: 2,800千円、間接経費: 840千円)
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キーワード | Raman imaging / Data standardadization / stemness markor / cell moprhology / molecular imaging / machine learning / Raman imaging / cancer stem cells |
研究開始時の研究の概要 |
The first target of this project will be to evaluate with hyperspectral Raman imaging the metabolic changes that occur when cancer cells are cultivated on hydrogels in comparison to usual samples. Especially lipid metabolism alteration and redox states will be clarified. The final target will be to explore the action of immune system cells, macrophages, in the genesis or the maintenance of cancer stem cells.
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研究実績の概要 |
During this fiscal year was investigated the action of PAMPS gel on myoblast cell models. It was found that Poly (2-acrylamido-2- methyl-1-propanesulphonic acid) hydrogel have a significant impact to induce an elevation of stemness markers such as Sox2, Nanog, and Oct3/4. This elevation of stemness markers have been linked to a change of cell morphology, meaning that cell that have a higher expression level tend to be more round and small whereas the one that have a low expression of stemness markers tend to be bigger and flat. This interesting fact showed that by staining
The first Raman images of the mentioned hydrogel-cell interaction system was performed with a high-speed slit-scanning Raman microscope. The quality of the measurement was performed with different metric such as signal-to-noise ratio, Laplacian operator and non-homogenous line illumination quantification. As a result the measurement protocol for this complex measurement provide high-quality data.
To exploit Hyperspectral Raman images on hydrogel substrate, two algorithms that allow to compare Data from different Raman devices and different dates of measurement was finalized. One algorithm is for single cell measurement. Another one is for 3D colony formation measurement.
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現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
The measurement of Raman image with hydrogel-cell substrate being slightly more complex than standard substrate measurement, a slight delay can be observed compared to what was originally planned.
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今後の研究の推進方策 |
The hydrogel substrate is a fascinating platform for investigating the complexity of live cells, and can ultimately lead to a better understanding of cancer cell behavior when subjected to varying mechanical properties, as hydrogels can be precisely tuned.
In this fiscal year, by tuning the mechanical properties of hydrogels, and combining Raman measurement with the two algorithms developed in the previous fiscal year for analyzing such data, I will elucidate the effect of mechanical constraints on the metabolic state of myoblast cell lines and cancer cell lines. This will provide a better understanding of the origin of the elevated cancer stemness markers observed on such substrates.
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