| 研究課題/領域番号 |
22K18368
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| 研究種目 |
挑戦的研究(開拓)
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| 配分区分 | 基金 |
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| 審査区分 |
中区分45:個体レベルから集団レベルの生物学と人類学およびその関連分野
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| 研究機関 | 筑波大学 |
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研究代表者 |
UTADA ANDREW 筑波大学, 生命環境系, 准教授 (90776626)
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| 研究分担者 |
McGlynn Shawn・E. 東京工業大学, 地球生命研究所, 准教授 (10751084)
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| 研究期間 (年度) |
2022-06-30 – 2025-03-31
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| 研究課題ステータス |
交付 (2022年度)
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| 配分額 *注記 |
25,870千円 (直接経費: 19,900千円、間接経費: 5,970千円)
2024年度: 2,990千円 (直接経費: 2,300千円、間接経費: 690千円)
2023年度: 10,660千円 (直接経費: 8,200千円、間接経費: 2,460千円)
2022年度: 12,220千円 (直接経費: 9,400千円、間接経費: 2,820千円)
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| キーワード | heterogeneity / metabolism / NanoSIMS / Single-cell tracking / Metaboloic activitity / Bacteria / Heterogeneity |
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| 研究開始時の研究の概要 |
A new theory of microbial population activity is needed to further our understanding of population dynamics and heterogeneity. In order to do this, we will lay the groundwork for this new theory by coherently combining heretofore separate domains: (i) single-cell tracking of bacteria using high spatio-temporal resolution imaging in time and in space; and (ii) NanoSIMS, which reveals anabolic activity at the single-cell level by imaging stable isotopes.
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| 研究実績の概要 |
In the past year, we have started by finding an appropriate research scientist. We have begun to identify a set of bacteria to use that are genetically tractable and widely applicable. This list currently includes Escherichia coli and Pseudomonas aeruginosa. We have focused on developing the two major thrusts of our targeted work: single cell tracking in confined conditions and the nano secondary ion mass spectrometry (nanoSIMS) process. We have already begun to optimized our cell tracking using publicly available machine learning algorithms combined with custom designed microfluidic chambers.
Simultaneously, we have developed microfabrication methods to use with hard glass substrates. We plan to combine these results soon to use with the local nanoSIMS facility.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
We are slightly delayed due to the slow start of the postdoc and the lengthy trainings needed to use the necessary equipment in local nano-platoform clean rooms. We expect to accelerate our testing in the coming months.
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| 今後の研究の推進方策 |
Our future work will be to combine our custom chambers, where we can track cells as they develop into colonies with protocols that are compatible with the nanoSIMS facilities. These facilities have stringent rules that require precise alignment. We are currently conducting some initial stable isotope tests, which we will be able to "fix" and analyze when we are able to secure time using the nanoSIMS equipment.
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