| 研究課題/領域番号 |
22K20535
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| 研究種目 |
研究活動スタート支援
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| 配分区分 | 基金 |
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| 審査区分 |
0501:物理化学、機能物性化学、有機化学、高分子、有機材料、生体分子化学およびその関連分野
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| 研究機関 | 岡山大学 |
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研究代表者 |
FERREPUJOL PILAR 岡山大学, 異分野融合先端研究コア, 特任助教 (50839008)
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| 研究期間 (年度) |
2022-08-31 – 2024-03-31
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| 研究課題ステータス |
完了 (2023年度)
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| 配分額 *注記 |
2,860千円 (直接経費: 2,200千円、間接経費: 660千円)
2023年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2022年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
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| キーワード | graphene oxide / reduced grapehene oxide / cryoprotectant / oocyte / vitrification / reduced graphene oxide / in vitro maturation / cryopreservation / nanocarbons / reactive oxygen species |
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| 研究開始時の研究の概要 |
Nanocarbons such as graphene and graphene oxide (GO) have multiple applications in the biological field. Because of their controllable surface chemistry, such as oxidation levels, and easy functionalization with other molecules, they can be used as ROS donors/quenchers.
This is of special interest in the field of germplasm cryopreservation, where the success is limited at the thawing stage, because excessive ROS production damages the cells. For this, I will study the application of graphene and GO during the thawing and recovery stages after cryopreservation to obtain high quality oocytes.
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| 研究実績の概要 |
The results indicated that when graphene oxide (GO) and reduced graphene oxide (rGO) were used during vitrification, they negatively affected the survival of cumulus-oocyte-complexes due to the strong affinity of the materials with the cell membranes. Furthermore, when the materials were added after thawing, their protective effect against reactive oxygen species could also not be clearly evaluated. The high reactivity of the surface of GO with the inorganic components of the culture media (especially with positive ions such as Na, K or Ca) could partly explain the reason why pristine GO and rGO were unable to successfully protect the oocytes from oxidative stress.
Further studies employing surface-modified GO are necessary to successfully use this material for cryopreservation.
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