| 研究課題/領域番号 |
22K20705
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| 研究種目 |
研究活動スタート支援
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| 配分区分 | 基金 |
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| 審査区分 |
0801:薬学およびその関連分野
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| 研究機関 | 北海道大学 |
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研究代表者 |
タン ロジャー・サルバシオン 北海道大学, 先端生命科学研究院, 助教 (10951759)
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| 研究期間 (年度) |
2022-08-31 – 2024-03-31
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| 研究課題ステータス |
中途終了 (2023年度)
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| 配分額 *注記 |
2,860千円 (直接経費: 2,200千円、間接経費: 660千円)
2023年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
2022年度: 1,430千円 (直接経費: 1,100千円、間接経費: 330千円)
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| キーワード | Exosome / Nanosome / Drug Delivery System / Nanoparticles / Exosome-nanosme / Cancer cells / Nanoparticle |
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| 研究開始時の研究の概要 |
Part 1: Exosome target organ determination.
Part 2: Nanosome construction and Drug loading.
Part 3: Efficiency testing of the novel platform.
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| 研究実績の概要 |
In the extracellular vesicles population produced by cells, exosomes only constitute a small portion of the population. This has been one of the limitations of studying exosomes. One of the optimization protocols necessary and employed in this research was to find a way to induce the production of a higher number of exosomes in cancer cells. After employing different strategies, we were able to successfully induce cancer cells to produce a higher number of exosomes by starving them. Production and isolation of exosomes from starved cancer cells were then optimized to yield a stably high number of exosomes. Starved cancer cells produced a higher number of exosomes (1.56e+15 particles per mL with an average particle size of 139.5 nm) compared to the fed cancer cells (1.51e+10 particles per mL with an average particle size of 106.5 nm). This finding enabled us to optimize the exosome production and yielded considerably more exosomes needed for the study.
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