| 研究課題/領域番号 |
22KF0065
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| 補助金の研究課題番号 |
22F20801 (2022)
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| 研究種目 |
特別研究員奨励費
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| 配分区分 | 基金 (2023)
補助金 (2022) |
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| 応募区分 | 外国 |
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| 審査区分 |
小区分49070:免疫学関連
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| 研究機関 | 千葉大学 |
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研究代表者 |
藤橋 浩太郎 (2023) 千葉大学, 医学部附属病院, 特任教授 (50820354)
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| 研究分担者 |
TSAI JIA-YUN 千葉大学, 医学部附属病院, 外国人特別研究員
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| 受入研究者 |
藤橋 浩太郎 (2022) 千葉大学, 医学部附属病院, 特任教授 (50820354)
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| 外国人特別研究員 |
TSAI JIA-YUN 千葉大学, 医科学研究所, 外国人特別研究員
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| 研究期間 (年度) |
2023-03-08 – 2024-03-31
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| 研究課題ステータス |
完了 (2023年度)
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| 配分額 *注記 |
1,200千円 (直接経費: 1,200千円)
2023年度: 200千円 (直接経費: 200千円)
2022年度: 1,000千円 (直接経費: 1,000千円)
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| キーワード | Vaccine / influenza virus / IgA / Protection / Nasal Vaccine / Influenza virus |
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| 研究開始時の研究の概要 |
We will assess whether PilVax nasal vaccine induces antigen (M2e)-specific antibody responses in both mucosal and systemic compartments. When significant titers of M2e-specific mucosal IgA and serum IgG antibody responses are noted, we will test the functional properties of the M2e-specific antibodies by challenging vaccinated mice with influenza virus strains (H1N1, e.g., A/California and H3N2, e.g., A/Guizhou) to confirm protective immunity.
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| 研究実績の概要 |
Age-matched female BALB/c mice were immunized with of PilVax vaccines (1 X 10^8 CFU, in a volume of 10 ul) presenting two tandem copies (M2e-M2e-PilVax) of the M2e peptide. The vaccines were administered on 3 consecutive days with additional 3 boosters given 2 weeks apart, for a total of 12 doses. One week after the final immunization (day 51 or 49), serum, bronchoalveolar lavage (BAL) nasal washes (NWs) and saliva were collected and subjected to M2e-specific ELISAs. Significantly increased levels of M2e-specific serum IgG and mucosal IgA antibody responses were noted. When vaccinated mice were challenged with influenza virus (H1N1, e.g., A/California) in order to confirm protective immunity, nasal PilVax immunized mice showed complete protection from the virus challenge.
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