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緑色果実のための蛍光に基づくラベルフリー技術を用いた超精密農業

研究課題

研究課題/領域番号 22KF0179
補助金の研究課題番号 21F21397 (2021-2022)
研究種目

特別研究員奨励費

配分区分基金 (2023)
補助金 (2021-2022)
応募区分外国
審査区分 小区分41040:農業環境工学および農業情報工学関連
研究機関京都大学

研究代表者

近藤 直 (2021, 2023)  京都大学, 農学研究科, 教授 (20183353)

研究分担者 HUANG ZICHEN  京都大学, 農学研究科, 外国人特別研究員
受入研究者 近藤 直 (2022)  京都大学, 農学研究科, 教授 (20183353)
外国人特別研究員 HUANG ZICHEN  京都大学, (連合)農学研究科(研究院), 外国人特別研究員
研究期間 (年度) 2023-03-08 – 2024-03-31
研究課題ステータス 完了 (2023年度)
配分額 *注記
2,200千円 (直接経費: 2,200千円)
2023年度: 600千円 (直接経費: 600千円)
2022年度: 1,100千円 (直接経費: 1,100千円)
2021年度: 500千円 (直接経費: 500千円)
キーワードimage / fluorescence / grading / green fruit / machine vision / grader / green fruits / fluorescence imaging / label-free / disease
研究開始時の研究の概要

There are two topics in this research. The first topic is to build an acoustic-based hybrid localization system for a greenhouse robot. Experiments will be conducted to evaluate its localization performance, including accuracy and Doppler shift to moving objects. The second topic is the application of fluorescence imaging-based sensing methods to the precision agriculture of green peppers. Early-stage diseases will be studied and detected using fluorescence images. Also, a label-free method will be established to track individual peppers.

研究実績の概要

We developed a fluorescence image acquisition system for analyzing the phenotype of green peppers. The fruits had some fluorescence substance whose excitation wavelength was 365 nm and emitted blue fluorescence, which can be captured by cameras within the visible light spectrum. Notably, the "Manganji sweet peppers" in Kyoto, Japan, represent a distinctive variety with significant demand for agricultural big data under their regional brand. In response to the need for individual green pepper traceability with big data, we identified fluorescence features in green pepper images that are imperceptible under natural light. Through mechanistic analysis, we determined that over 93% of these fluorescence features naturally arise during the growth process and remain stable. Leveraging feature point matching algorithms, we constructed a label-free tracking system that enabled the traceability of individual green peppers, achieving an 83% accuracy rate in line with commercial application standards. The integration of the indoor positioning system and fluorescence imaging system aligns with the overarching objective of this research, which is to elevate precision agriculture to a level where plant-to-plant accuracy is attainable. We have authored or co-authored 6 SCI papers for showing the new findings as well as presentations at 5 international academic conferences. These publications and conference contributions play a crucial role in amplifying the impact of our research endeavors.

報告書

(3件)
  • 2023 実績報告書
  • 2022 実績報告書
  • 2021 実績報告書
  • 研究成果

    (5件)

すべて 2023

すべて 雑誌論文 (4件) (うち査読あり 2件) 学会発表 (1件)

  • [雑誌論文] Macroscopic and Microscopic Characterization of Fluorescence Properties of Multiple Sweet Pepper Cultivars (Capsicum Annuum L.) Using Excitation-Emission Matrix and UV Induced Fluorescence Imaging.2023

    • 著者名/発表者名
      Huang, Z.; Takemoto, T.; Omwange, K.A.; Saito, Y.; Kuramoto, M.; Kondo, N
    • 雑誌名

      Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

      巻: 288 ページ: 122094-122094

    • DOI

      10.1016/j.saa.2022.122094

    • 関連する報告書
      2023 実績報告書
  • [雑誌論文] Investigating the characteristics of fluorescence features on sweet peppers using UV light excitation2023

    • 著者名/発表者名
      Huang Zichen、Takemoto Tetsuyuki、Saito Yoshito、Omwange Ken Abamba、Konagaya Keiji、Hayashi Takahiro、Kondo Naoshi
    • 雑誌名

      Photochemical & Photobiological Sciences

      巻: 22 号: 10 ページ: 2401-2412

    • DOI

      10.1007/s43630-023-00459-5

    • 関連する報告書
      2023 実績報告書
    • 査読あり
  • [雑誌論文] Label-free technology for traceable identification of single green pepper through features in UV fluorescent images2023

    • 著者名/発表者名
      Takemoto Tetsuyuki、Huang Zichen、Omwange Ken Abamba、Saito Yoshito、Konagaya Keiji、Suzuki Tetsuhito、Ogawa Yuichi、Kondo Naoshi
    • 雑誌名

      Computers and Electronics in Agriculture

      巻: 211 ページ: 107960-107960

    • DOI

      10.1016/j.compag.2023.107960

    • 関連する報告書
      2023 実績報告書
    • 査読あり
  • [雑誌論文] Kondo, N. Monitoring Strawberry (Fragaria × Ananassa) Quality Changes during Storage Using UV-Excited Fluorescence Imaging.2023

    • 著者名/発表者名
      Huang, Z.; Omwange, K.A.; Saito, Y.; Kuramoto, M.; Kondo, N.
    • 雑誌名

      Kondo, N. Monitoring Strawberry (Fragaria × Ananassa) Quality Changes during Storage Using UV-Excited Fluorescence Imaging.

      巻: 353 ページ: 111553-111553

    • 関連する報告書
      2023 実績報告書
  • [学会発表] Research progress of Japanese harvesting robot in facility agriculture,China, Invitational Lecture2023

    • 著者名/発表者名
      Zichen HUANG
    • 学会等名
      The 7th World Intelligence Congress Intelligent Agriculture Summit Forum & International Conference on Intelligent Agriculture (ICIA2023)
    • 関連する報告書
      2023 実績報告書

URL: 

公開日: 2022-02-08   更新日: 2024-12-25  

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