| 研究課題/領域番号 |
22KF0208
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| 補助金の研究課題番号 |
22F22050 (2022)
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| 研究種目 |
特別研究員奨励費
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| 配分区分 | 基金 (2023)
補助金 (2022) |
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| 応募区分 | 外国 |
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| 審査区分 |
小区分90110:生体医工学関連
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| 研究機関 | 京都大学 |
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研究代表者 |
角五 彰 京都大学, 理学研究科, 教授 (10374224)
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| 研究分担者 |
NASRIN SYEDA 京都大学, 理学研究科, 外国人特別研究員
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| 研究期間 (年度) |
2023-03-08 – 2025-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
2,300千円 (直接経費: 2,300千円)
2024年度: 800千円 (直接経費: 800千円)
2023年度: 800千円 (直接経費: 800千円)
2022年度: 700千円 (直接経費: 700千円)
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| キーワード | kinesin / Dynein / Microtubule / Mechanical stress / Mechano-transducer / Kinesin |
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| 研究開始時の研究の概要 |
The current research hypothesizes that microtubules are mechanotransducers of mechanical stress. In this study, a comprehensive understanding of the issue will be made. The research will explore if the effect of the mechanically deformed microtubules is true regardless of the motor protein species.
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| 研究実績の概要 |
The current research hypothesizes that microtubules are mechanotransducers of mechanical stress. In this study, a comprehensive understanding of the issue will be made. Last year the modulation of cargo transport in response to mechanical stress on the microtubule is a universal phenomenon independent of the motor protein by using kinesin was verified. Kinesin-driven transport of fluorescent quantum dots was carried out on microtubules fixed on a stretchable substrate. The effect of the mechanical stress on the cargo transport was systematically evaluated by applying compressive strain or tensile strain using the microstretcher chamber developed previously. Parameter analysis e.g., run length, velocities, pause frequencies, etc., from the fluorescence microscopy observation was done.
This year, we also verified if the modulation of cargo transport in response to mechanical stress is recovered if the applied stress on the microtubule was withdrawn. For this research, I will first apply compressive stress on the microtubule using my micro-stretcher and release the stress. Then I observed dynein or kinesin-driven cargo transport and compare it with the case of undeformed microtubules to explore the restoration of the transport dynamics.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Because the research is progressing as planned, it was concluded that the project was on track.
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| 今後の研究の推進方策 |
Next year, we will summarize the following questions: (1) How does the mechanical stress-induced deformation of microtubules affect kinesin-driven cargo transport? (2) Is the cargo transport dynamics restored when applied stress on the microtubule is withdrawn? (3) Can the altered transport dynamics of dynein be theoretically explained by techniques like molecular dynamics simulation? We are also planning to propose a mechanism that explain the relationship between mechanical stress and the dynamics of kinesin.
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