| 研究課題/領域番号 |
22KF0287
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| 補助金の研究課題番号 |
21F21353 (2021-2022)
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| 研究種目 |
特別研究員奨励費
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| 配分区分 | 基金 (2023)
補助金 (2021-2022) |
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| 応募区分 | 外国 |
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| 審査区分 |
小区分27040:バイオ機能応用およびバイオプロセス工学関連
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| 研究機関 | 九州大学 |
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研究代表者 |
井嶋 博之 九州大学, 工学研究院, 教授 (10274515)
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| 研究分担者 |
SHAFIQ MUHAMMAD 九州大学, 工学研究院, 外国人特別研究員
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| 研究期間 (年度) |
2023-03-08 – 2024-03-31
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| 研究課題ステータス |
完了 (2023年度)
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| 配分額 *注記 |
2,200千円 (直接経費: 2,200千円)
2023年度: 900千円 (直接経費: 900千円)
2022年度: 1,000千円 (直接経費: 1,000千円)
2021年度: 300千円 (直接経費: 300千円)
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| キーワード | Hybrid vascular graft / Decellularized ECM / Polycaprolactone / Nanofibrous membrane / Tubular scaffold / Electrospun membrane / Nanofibrous Membrane |
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| 研究開始時の研究の概要 |
The aim of this research is to decipher the role of immunomodulation for engineering synthetic vascular grafts, which will be accomplished by exploiting decellularized extracellular matrix (dECM) and different immunomodulatory cues. Cell free vascular grafts may offer an invaluable platform for vascular tissue engineering. In this study, PLCL / dECM based hybrid vascular grafts will be developed by electrospinning. Furthermore, immobilize bioactive cues will elucidate the role of immunomodulatory for vascular tissue repair.
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| 研究実績の概要 |
Artificial nerve guidance conduits (NGCs) consisting of core/shell type electrospun fibers using brain-derived extracellular matrix (B-ECM) have been developed and their effectiveness has been demonstrated. Their details are as follows.
Fabrication of core/shell type electrospun fibers: B-ECM was blended along with gelatin to fabricate polycaprolactone/gelatin (PCL/Gel)-based core/shell type fibers. At this time, the aligned fibers using a rotating collector were also obtained.
Evaluation by cell culture: PC12 cells were used as neural model cells. In the nonwoven fabric made of the electrospun ECM described above, the effect of promoting the elongation of neurites and the elongation of neurons on the aligned fibers was confirmed. NGF was immobilized on the fibers because neurotrophic factors and growth factors can affect cell differentiation and tissue repair. Here, nerve growth factor (NGF) was able to be immobilized on the fibers via glycosaminoglycans, which are constituents of B-ECM.
Fabrication and evaluation of nerve guidance conduits: NGCs were fabricated by amassing tubular scaffolds and core/shell type aligned electrospun fibers. Physico-chemical properties of NGCs, including tensile testing and suture strength retention were sufficient for the application. Biocompatibility of tubular scaffolds was also confirmed. The NGCs were transplanted into sciatic nerve defects, and suppression of neurite outgrowth and cell infiltration from the surrounding area was achieved.
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