| 研究課題/領域番号 |
22KJ0427
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| 補助金の研究課題番号 |
22J20479 (2022)
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| 研究種目 |
特別研究員奨励費
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| 配分区分 | 基金 (2023)
補助金 (2022) |
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| 応募区分 | 国内 |
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| 審査区分 |
小区分64030:環境材料およびリサイクル技術関連
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| 研究機関 | 筑波大学 |
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研究代表者 |
ALAWI ASEEL 筑波大学, 理工情報生命学術院, 特別研究員(DC1)
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| 研究期間 (年度) |
2023-03-08 – 2025-03-31
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| 研究課題ステータス |
交付 (2023年度)
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| 配分額 *注記 |
2,500千円 (直接経費: 2,500千円)
2024年度: 800千円 (直接経費: 800千円)
2023年度: 800千円 (直接経費: 800千円)
2022年度: 900千円 (直接経費: 900千円)
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| キーワード | glycerol / 1,3-propanediol / 3-hydroxypropionic acid / fermentation / metabolic engineering / overexpression / Citrobacter braakii |
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| 研究開始時の研究の概要 |
This research focuses on the metabolic engineering of microorganisms for the production of useful chemical from glycerol.
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| 研究実績の概要 |
To increase 1,3-propandiol production titer we aimed at enhancing glycerol consumption of TB-96-dLF by overexpression related enzymes. The overexpression of gdh reactivation factor was examined. Fermentation experiment of constructed strain showed higher 1,3-PD productivity but lower bacterial growth and a long lag phase until the bacteria entered exponential phase.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
We expect noticeable improvement of bacterial growth as well as 1,3-propanediol production from glycerol when by enhancing glycerol consumption through overexpression of gdh or its reactivation factor. Our obtained results showed a declined bacterial growth and long lag phase until the bacterial entered exponential phase.
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| 今後の研究の推進方策 |
Since overexpression system using pUG19 plasmid was established, we plan to investigate the production of 3-hydroxypropionic acid from glycerol by overexpressing native or heterologous genes related to this pathway.
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