| 研究課題/領域番号 |
22KJ0894
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| 補助金の研究課題番号 |
22J13110 (2022)
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| 研究種目 |
特別研究員奨励費
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| 配分区分 | 基金 (2023)
補助金 (2022) |
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| 応募区分 | 国内 |
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| 審査区分 |
小区分43010:分子生物学関連
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| 研究機関 | 国立研究開発法人理化学研究所 (2023)
東京大学 (2022) |
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研究代表者 |
韓 佩恂 国立研究開発法人理化学研究所, 開拓研究本部, 特別研究員(PD)
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| 研究期間 (年度) |
2023-03-08 – 2024-03-31
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| 研究課題ステータス |
完了 (2023年度)
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| 配分額 *注記 |
2,300千円 (直接経費: 2,300千円)
2023年度: 1,100千円 (直接経費: 1,100千円)
2022年度: 1,200千円 (直接経費: 1,200千円)
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| キーワード | Ribosome / Translation |
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| 研究開始時の研究の概要 |
During translation process, genetic information in mRNA transcripts is converted into protein. It is known that cells utilize specific mechanism to regulate gene expression at translation level. One case during translation is when a translating ribosome collides into a downstream ribosome, forming a ribosome collision. However, it remains unclear how ribosome collisions are utilized by cells to regulate translation. Thus, this project focuses on investigating the endogenous features of ribosome collision to further dissect the role of collided ribosomes during translation.
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| 研究実績の概要 |
Translation, as a fundamental step in gene expression, requires extensive regulation. Recent studies have shown that the trigger of ribosome-associated quality control is the collision between two translating ribosomes. However, it is not clear whether endogenous mRNA sequences could also trigger quality control.
By deep-sequencing the ribosome footprints protected by collided ribosomes prior to rescue, we determined a specific feature in the mRNA sequence that contributed to its recognition by ribosome-associated quality control. Further analysis through reporter assay also validated our findings from the analysis.
These results combined provided crucial evidences regarding co-translational quality control pathways in higher eukaryotic cells.
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